【摘要】 目的 探究芜菁中性多糖对A549小鼠移植瘤模型肿瘤微环境及凋亡相关蛋白、细胞因子水平的影响。方法 实验采用酶联免疫法、HE染色法、免疫组化法,检测半胱氨酸天冬氨酸蛋白酶3 (cysteinyl aspartate specific proteinase 3, caspase-3)、白细胞介素2 (interleukin-2, IL-2)、白细胞介素6 (interleukin-6, IL-6)、白细胞介素12 (interleukin-12, IL-12)和肿瘤坏死因子-α (tumor necrosis factor-α, TNF-α)含量及B淋巴细胞瘤-2 (B-cell lymphoma-2, Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein, Bax)相对表达水平,计算免疫器官指数、抑瘤率。结果 芜菁中性多糖中、高剂量组能使A549荷瘤小鼠肿瘤组织caspase-3蛋白含量显著增加(P<0.01);使小鼠血清中IL-2、IL-6、IL-12、TNF-α含量升高(P<0.01);Bcl-2蛋白表达水平降低,Bax蛋白表达水平升高(P<0.01)。结论 芜菁中性多糖对人肺腺癌细胞A549生长具有抑制作用,可提高A549荷瘤小鼠的脾脏指数和胸腺指数,下调Bcl-2蛋白,上调Bax蛋白的表达,减轻荷瘤小鼠脏器病理损伤。更多还原

【Abstract】 Objective To study the effects of Brassica rapa L. neutral polysaccharide on apoptosis-related polypeptide and cytokines of transplanted mice model of A549 carcinoma in vitro. Methods Enzyme-linked immunosorbent assay, HE staining and immunohistochemistry were used to detect cysteinyl aspartate specific proteinase 3(caspase-3), interleukin-2(IL-2), interleukin-6(IL-6), interleukin-12(IL-12), and tumor necrosis factor-α(TNF-α)content and B-cell lymphoma-2(Bcl-2), the relative expression level of Bcl-2 associated X protein(Bax), and the immune organ index and tumor inhibition rate were calculated. Results The moderate and high dose groups of Brassica rapa L. neutral polysaccharide could significantly increase the caspase-3 protein content in A549 tumor-bearing mice(P<0.01). The content of IL-2, IL-6, IL-12 and TNF-α in serum of mice were increased(P<0.01). The expression level of Bcl-2 protein was decreased, while that of Bax protein was increased(P<0.01). Conclusion Brassica rapa L. neutral polysaccharides can inhibit growth of A549 human lung adenocarcinoma cells, increase the spleen index and thymus index of A549 tumor-bearing mice, increase the expression of Bax protein through downregulation of Bcl-2 protein,and reduce the pathological damage in tumor-bearing mice.更多还原