【摘要】 目的 探究西番莲果皮花色苷（peel of passion fruits anthocyanins,PPFA）对H₂O₂诱导人正常肝细胞L02氧化损伤的抑制作用。方法 采用噻唑蓝[3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide,MTT]法建立H₂O₂诱导L02细胞氧化损伤的模型,通过测定细胞丙二醛（malondialdehyde,MDA）、乳酸脱氢酶（lactate dehydrogenase,LDH）、超氧化物歧化酶（superoxide dismutase,SOD）、过氧化氢酶（catalase,CAT）和谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）指标,观察PPFA对细胞氧化损伤的抑制作用,结合非靶向代谢组学显著差异代谢物分析,探讨其抗氧化机制。结果 PPFA体外清除1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基的半抑制浓度（half maximal inhibitory concentration,IC50）值为34.62μg/m L,有一定的羟自由基、超氧阴离子自由基清除能力。细胞实验表明,PPFA能够显著提高H₂O₂损伤的L02细胞总抗氧化力（P<0.05）,并显著降低损伤细胞中MDA生成量和LDH的释放量（P<0.05）;与损伤组相比,PPFA各剂量组SOD活性均显著升高（P<0.05）,中剂量组CAT与低剂量组GSH-Px的活性显著升高且达空白组水平（P<0.05）。代谢组学分析表明,与损伤组相比,PPFA处理后细胞存在23种显著差异代谢物,涉及到谷胱甘肽代谢、胆汁分泌、铁死亡和脂肪酸生物合成途径。结论 PPFA对H₂O₂诱导L02细胞的氧化损伤有抑制作用,表现出显著的细胞抗氧化活性。更多还原

【Abstract】 Objective To investigate the inhibitory effect of peel of passion fruits anthocyanins（PPFA） on H₂O₂-induced oxidative damage in human normal liver cells L02. Methods A model of H₂O₂-induced oxidative damage in L02 cells was established by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）method,and the inhibitory effect of PPFA on cellular oxidative damage was observed by measuring cellular malondialdehyde（MDA）,lactate dehydrogenase（LDH）,superoxide dismutase（SOD）,catalase（CAT） and glutathione peroxidase（GSH-Px） indexes,combined with non-targeted metabolomics significantly different metabolite analysis to explore its antioxidant mechanism. Results The half maximal inhibitory concentration（IC50）value of 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical scavenging by PPFA in vitro was 34.62 μg/mL,and it had certain scavenging ability hydroxyl radicals and superoxide anion radicals. Cellular experiments showed that PPFA could significantly increase the total antioxidant power of H₂O₂-induced L02 cells（P<0.05）,and significantly decrease the MDA production and LDH release in the damaged cells（P<0.05）; compared with the damaged group,the SOD activity of PPFA was significantly increased in all dose groups（P<0.05）,and the activity of CAT in the middle dose group and GSH-Px in the low dose group were significantly increased and reached the normal level（P<0.05）. Metabolomic analysis showed that there were 23 kinds of significantly different metabolites in PPFA-treated cells compared with the damaged group,involving glutathione metabolism,bile secretion,ferroptosis and fatty acid biosynthesis pathways. Conclusion PPFA can inhibit the H₂O₂-induced oxidative damage in L02cells,showing significant cellular antioxidant activity.更多还原