【摘要】 目的 采用网络药理学方法探讨益肾蠲痹丸治疗类风湿关节炎（RA）的分子作用机制。方法 采用中药系统药理学数据库与分析平台（TCMSP）、中医药整合药理学研究平台（TCMIP）、本草组鉴（HERB）和中医药百科全书数据库（ETCM）获取益肾蠲痹丸的有效成分，并借助SwissTargetPrediction数据库对有效成分的作用靶点进行预测；在人类基因综合数据库（GeneCards）、人类疾病相关基因与突变位点信息数据库（DisGeNET）、在线人类孟德尔遗传数据库（OMIM）、药物靶标数据库（TTD）中搜索RA相关基因。将益肾蠲痹丸的作用靶点和与RA相关靶点进行映射交集，获得二者的交集靶点，借助STRING数据库构建蛋白互作（PPI）网络；采用Cytoscape 3.7.2软件筛选出核心靶点，采用Cytoscape 3.9软件进行基因本体（GO）、京都基因与基因组百科全书（KEGG）信号通路分析。结果 共获得益肾蠲痹丸治疗RA的有效成分185个，其中关键成分为山柰酚、木犀草素、槲皮素和黄藤素，核心作用靶点为肿瘤坏死因子（TNF）、白介素-6(IL-6)、白介素-1β(IL-1β)、血管内皮生长因子A(VEGFA)、信号转导及转录激活蛋白3(STAT3)基因等。KEGG信号通路主要涉及TNF、丝裂原活化蛋白激酶（MAPK）、白介素-17(IL-17)、VEGF、核因子-κB(NF-κB)信号通路及RA疾病信号通路等。结论 益肾蠲痹丸治疗RA具有多成分、多靶点、多通路协同作用优势，可通过调控相关信号通路调节机体免疫功能、影响细胞凋亡、抑制炎症反应。更多还原

【Abstract】 Objective To investigate the molecular mechanism of Yishen Juanbi Wan in the treatment of rheumatoid arthritis(RA) based on a network pharmacology approach. Methods Active components of Yishen Juanbi Wan were obtained from the TCMSP、TCMIP、HERB and ETCM databases, and their targets of active components were predicted using the SwissTarget Prediction database. RA-related genes were retrieved from databases including GeneCards, DisGeNET、OMIM and TTD databases. The targets of Yishen Juanbi Wan and the targets related to RA were mapped to obtain the intersection targets, PPI networks were constructed with the help of STRING database, the key targets of Yishen Juanbi Wan in treating RA were screened using Cytoscape 3.7.2 software, and GO and KEGG enrichment analyses were performed with Cytoscape 3.9 software. Results A total of 185 active components were obtained for the treatment of RA, including those key components like kaempferol, luteolin, quercetin and palmatine, and the core gene targets were TNF, IL-6, IL-1β, VEGFA, and STAT3, etc. KEGG analysis showed that the main signaling pathways involved were TNF, MAPK, IL-17, VEGF, NF-κB, and rheumatoid arthritis. Conclusion With the advantages of multi-component, multi-target and multipathway synergistic effects in the treatment of RA, Yishen Juanbi Wan can effectively regulate the body’s immune function, affect apoptosis and inhibit inflammatory response by regulating relevant signaling pathways.更多还原