【摘要】 研究从大规模单细胞转录组测序数据出发，获得结直肠癌(colorectal cancer, CRC)中肠道内分泌细胞(enteroendocrine cell, EEC)的单细胞转录组图谱；对此转录组测序数据作差异表达、功能以及拟时序分析，结果提示肿瘤微环境中EEC发育与激素合成/分泌相关功能不全有关；结合肿瘤基因组图谱(The Cancer Genome Atlas, TCGA)和肿瘤药敏多组学数据库(Genomics of Drug Sensitivity in Cancer, GDSC)中CRC的bulk转录组测序数据、随访信息和药物测试数据，筛选出对CRC患者生存具有积极影响的生物学标志物——胰高血糖素(glucagon,GCG)基因；利用肿瘤细胞系药物测试数据探究了GCG基因在CRC治疗中的潜在应用价值。该研究为CRC的治疗提供了新的思路。更多还原

【Abstract】 Single-cell transcriptome atlas of enteroendocrine cells(EEC) in colorectal cancer(CRC) was obtained from large-scale single-cell RNA sequencing(scRNA-seq) datum. Differential expression, function, and pseudotime analysis of EEC scRNA-seq datum suggest that EEC development is related to hormone synthesis/secretion function deficiencies in tumor microenvironment. Analysis of bulk RNA sequencing datum, follow-up information, and drug testing datum from The Cancer Genome Atlas(TCGA) and Genomics of Drug Sensitivity in Cancer(GDSC) datum of CRC reveal that the biomarker glucagon(GCG) has a positive impact on the survival of CRC patients. The potential application value of GCG gene in the treatment of CRC was explored by means of datum from tumor cell line drug tests. The results of this study provide new insights for the treatment of CRC.更多还原