【摘要】 目的 ：通过在镁锌钆合金（MZG）膜表面形成乙二醇化聚癸二酸甘油酯/β-磷酸三钙（PEGS/β-TCP）涂层，制备PEGS/β-TCP改性镁合金（PEGS/β-TCP/MZG）膜，使用材料浸提液对PEGS/β-TCP/MZG复合膜的成骨诱导活性和免疫调节性能进行评价。方法：采用溶胶凝胶法在MZG膜表面制备PEGS/β-TCP涂层，制备PEGS/β-TCP/MZG复合膜，并与PEGS/β-TCP和MZG膜进行对比，检测其形貌、成分和亲水性。检测材料浸提3天后的镁离子释放量和pH值。体外细胞实验观察成骨细胞MC3T3-E1细胞在浸提液中的增殖活性、碱性磷酸酶（alkaline phosphatase,ALP）和矿化结节形成情况，以及RAW264.7巨噬细胞在浸提液中的增殖活性、极化和细胞内相关因子的表达。采用GraphPad Prism 9.0软件包对数据进行统计学分析。结果：制备出PEGS/β-TCP涂层与MZG膜紧密嵌合的PEGS/β-TCP/MZG复合膜，具有良好亲水性，可缓慢降解。细胞实验结果显示，PEGS/β-TCP/MZG复合膜浸提液对MC3T3-E1和巨噬细胞的增殖活性无明显影响。PEGS/β-TCP/MZG组显著增强MC3T3-E1的ALP表达和矿化结节形成，PEGS/β-TCP组和PEGS/β-TCP/MZG组在巨噬细胞极化模式上虽无明显差异，但PEGS/β-TCP/MZG膜在PEGS/β-TCP涂层的免疫调节基础上，可进一步降低炎症相关肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)表达，提高成骨相关转化生长因子β(transforming growth factor-β,TGF-β)表达。结论：PEGS/β-TCP复合膜改性的MZG可为骨组织工程的发展提供新的材料选择。更多还原

【Abstract】 PURPOSE: To prepare PEGS/β-TCP modified magnesium alloy(PEGS/β-TCP/MZG) membranes by forming a glycolated poly(sebacate)/β-tricalcium phosphate(PEGS/β-TCP) coating on the surface of magnesium-zinc-gadolinium alloy(MZG) membranes, and to evaluate the osteogenic induction activity and immunomodulatory properties of PEGS/β-TCP/MZG using the material extract medium. METHODS: PEGS/β-TCP coating was prepared on the surface of MZG by solvent method, and the PEGS/β-TCP/MZG membrane was fabricated and compared with PEGS/β-TCP and MZG to examine the morphology, composition, and hydrophilicity. The amount of magnesium ions released and the pH value of the materials were tested after 3 days of immersion. The cell viability and osteogenic differentiation of MC3T3 cells induced by extract medium were investigated by CCK-8 assay, ALP and mineralized nodule staining. The cell viability and polarization of RAW cells induced by extract medium were then investigated. The expression of macrophage-secreted cytokines was examined by PCR analysis. GraphPad Prism 9.0 software package was used for statistical analysis. RESULTS: PEGS/β-TCP/MZG membranes with PEGS/β-TCP coating tightly embedded with MZG were successfully fabricated, and the material had good hydrophilicity. The results of degradation experiments indicated that the PEGS/β-TCP coating effectively slowed down the degradation rate of MZG, leading to a lower pH value and concentration of Mg2+ion in the extract medium of PEGS/β-TCP/MZG group. The results of in vitro cell experiments showed that PEGS/β-TCP/MZG had no significant effect on the proliferation activity of both MC3T3-E1 and macrophages. PEGS/β-TCP/MZG significantly enhanced the expression of ALP and mineralized nodule staining in MC3T3-E1. Although there was no significant difference in macrophage polarization pattern between PEGS/β-TCP and PEGS/β-TCP/MZG groups, PEGS/β-TCP/MZG further reduced inflammation based on the immunomodulation of PEGS/β-TCP coating related TNF-α expression and increased osteogenesis related TGF-β expression. CONCLUSIONS: MZG membrane modified by PEGS/β-TCP may provide a new material option for the development of bone tissue engineering.更多还原