【摘要】 我国是设施农业大国，聚乙烯(Polyethylene, PE)农用地膜大量使用，而且PE在环境中较难降解。为了得到能够高效降解PE的菌种资源，以PE地膜为唯一碳源，采用富集培养法从设施大棚土壤中筛选出一株高效的PE降解菌株，命名为BP1菌株，该菌株对PE农用地膜的降解率达9.99%。利用SEM电镜观察到BP1降解菌降解处理后PE地膜表面出现凹槽、生物侵蚀等现象。FTIR光谱仪分析表明，PE地膜表面发生C-H伸缩振动峰的削弱与酯羰基指数的降低。通过形态学观察和分子生物学鉴定，BP1菌株为枯草芽孢杆菌(Bacillus subtilis sp.)。该菌能显著提高PE地膜的降解速率，这为缓解废弃农用地膜污染问题，改善农田生态环境提供理论支撑。更多还原

【Abstract】 In the facility agriculture of our country, polyethylene(PE) film is widely used, which is difficult to degrade in the environment.In order to obtain strain resources capable of degrading PE,a highly efficient PE degrading strain BP1 was screened out from greenhouse soil by using PE mulch as the only carbon source and enrichment culture method.The degradation rate of the strain for PE film reached 9.99%.SEM and FTIR analysis showed that some grooves and bioerosion occurred on the surface of plastic film after BP1 degradation, and the C-H stretching vibration peak weakened and the ester carbonyl index decreased on the surface of plastic film.The strain BP1 was identified as Bacillus subtilis by morphological observation and molecular biological identification.The bacteria significantly increased the degradation rate of PE mulching film, which provided theoretical support for alleviating pollution of discarded agricultural mulching film and improving farmland ecological environment.更多还原