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桤木Genomic-SSR与EST-SSR分子标记的差异分析

Genetic Differences Revealed by Genomic-SSR and EST-SSR Markers in Alnus cremastogyne

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【作者】 王泽亮杨勇智杜晋城陈炙黄振郭洪英

【Author】 WANG Zeliang;YANG Yongzhi;DU Jincheng;CHEN Zhi;HUANG Zhen;GUO Hongying;Sichuan Academy of Forestry;Sichuan Academy of Grassland Sciences;

【机构】 四川省林业科学研究院四川省草原科学研究院

【摘要】 桤木(Alnus cremastogyne)为非豆科固氮树种,也是我国最重要的一个桤木属特有种,具有重要的生态功能。本文分析了桤木基因组SSR与EST-SSR两种来源标记的遗传差异,结果显示EST-SSR的平均等位基因数与平均有效等位基因数高于Genomic-SSR,而对平均观察杂合度与平均期望杂合度来说,Genomic-SSR高于EST-SSR,聚类分析显示Genomic-SSR与EST-SSR在小的分群上有差异,说明Genomic-SSR与ESTSSR标记在解析遗传多样性与遗传关系方面有一定差异,综合两种标记可以获得更加客观的结果。

【Abstract】 As a non-leguminous and nitrogen-fixing tree species, Alnus cremastogyne is also the most important endemic species of Alnus in China, which has important ecological functions. In this paper, the genetic differences of Genomic-SSR and EST-SSR markers in Alnus cremastogyne genome were analyzed. The results showed that the average number of alleles and the average number of effective alleles with EST-SSR were higher than those of genomic-SSR, while the average observed heterozygosity and average expected heterozygosity were higher than those of EST-SSR. Cluster analysis showed that there were differences between genomic-SSR and EST-SSR in small groups,which indicated that there were some differences between Genomic-SSR and EST-SSR in analyzing genetic diversity and genetic relationship to a certain extent, and more objective results could be obtained by combining the two marker methods.

【基金】 世界银行贷款长江流域上游森林生态系统恢复项目(2019-510000-02-01-400761);四川省科技计划项目(2021YFYZ0032)
  • 【文献出处】 四川林业科技 ,Journal of Sichuan Forestry Science and Technology , 编辑部邮箱 ,2023年04期
  • 【分类号】S792.14
  • 【下载频次】4
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