【摘要】 建立乳粉中6S-5-甲基四氢叶酸钙的反相高效液相色谱分析方法。使用三氯乙酸溶液沉淀蛋白，抗坏血酸溶液加热提取后，以C₁₈反相色谱柱分离，三氟乙酸溶液（0.1%）-甲醇为流动相，采用梯度方式洗脱，检测波长280 nm，单标法定量。结果表明：乳粉样品中6S-5-甲基四氢叶酸钙的定量限为150μg/100 g，检出限为50μg/100 g；乳粉中6S-5-甲基四氢叶酸钙的添加量为150～600?μg/100 g时，加标回收率为95.0%～109.1%，相对标准偏差为1.26%～4.04%。本方法操作简便、准确、回收率高、重复性好，适用于乳粉中6S-5-甲基四氢叶酸钙的含量测定。更多还原

【Abstract】 A reversed-phase high performance liquid chromatography （RP-HPLC） method was established for the determination of 6S-5-methyltetrahydrofolate calcium in milk powder.After protein precipitation with trichloroacetic acid solution,samples were extracted with ascorbic acid solution in a hot water bath,and the extract was separated on a C₁₈ reversed-phase column by gradient elution using a mobile phase composed of 0.1%trifluoroacetic acid solution and methanol,and the detection wavelength was set at 280 nm.Quantitative analysis was carried out by the single standard method.The results showed that the limit of quantification and detection of 6S-5-methyltetrahydrofolate calcium in milk powder samples were 150 and 50μg/100 g,respectively.The recoveries for samples spiked at levels ranging from 150 to600μg/100 g were 95.0%–109.1%with relative standard deviations （RSDs） between 1.26%and 4.04%.This method is simple,accurate,with high recovery and reproducibility,and suitable for the determination of 6S-5-methyltetrahydrofolate calcium in milk powder.更多还原