【摘要】 为确定获得软骨素裂解酶产生菌的分类地位及提高菌株产酶水平，通过形态学和16S rRNA基因序列分析鉴定菌株PL-410，并采用单因素试验、Plackett-Burman试验、最陡爬坡试验和Box-Behnken响应面试验优化菌株PL-410的产酶条件。结果表明，菌株PL-410为假节杆菌属（Pseudarthrobacter sp.），命名为假节杆菌PL-410；优化的最佳培养基为牛软骨硫酸软骨素添加量7.5 g/L、胰蛋白胨添加量2.5 g/L、NaCl添加量5 g/L、KH2PO4添加量0.3 g/L、培养基初始pH 6.53，最佳发酵条件为接种量2.98%、摇瓶装液量8%、摇床转速160 r/min、发酵温度24℃、发酵时间27.63 h，在此条件下软骨素裂解酶的活力（2 531.765 U/L）比优化前（85.229 U/L）提高28.7倍，可更有效地降解牛软骨硫酸软骨素。更多还原

【Abstract】 In order to determine its taxonomic status and to improve its enzyme production level, the chondroitinaseproducing strain PL-410 was identified based on morphology and 16S rRNA gene sequence analysis, and the fermentation conditions for chondroitinase production by this strain were optimized by single factor experiments, Plackett-Burman design,the steepest ascent method and Box-Behnken design combined with response surface methodology. The results showed that the strain PL-410 was identified as Pseudarthrobacter sp. The optimal medium was composed of chondroitin sulfate from bovine cartilage 7.5 g/L, tryptone 2.5 g/L, NaCl 5 g/L, KH2PO4 0.3 g/L, and initial pH 6.53. The optimal fermentation conditions were as follows: inoculum volume 2.98%, medium volume in shaking flasks 8%, shaker rotation speed 160 r/min,fermentation temperature 24 ℃, and fermentation time 27.63 h. The chondroitinase activity under these conditions was 2 531.765 U/L, which was 29.7 times higher than that before optimization(85.229 U/L).更多还原