【摘要】 橡胶树棒孢霉落叶病（Corynespora leaf fall disease, CLFD）是全球主要植胶国最为严重的叶部病害之一，可造成严重的产量和经济损失，而抗病种质鉴选与创制利用是该病最为有效的防治策略。本研究对云研277-5×IAN 873、RRIC103×热研8-79和云研277-5×热垦525三个杂交组合的821份F₁代群体进行了抗棒孢霉落叶病的评价，明确了F₁代群体的抗病性水平，并从符合正态分布的2个杂交组合中筛选出32份候选F₁代单株进行芽接，再分别利用3个亚型的多主棒孢病菌和2种评价方法对候选F₁代无性系种苗进行抗病性复筛，最终获得5份抗病性较好的F₁代新种质。通过对5份抗病新种质防御酶活性的测定，以及抗病相关基因表达特性的分析，进一步证实了5份抗病新种质与多主棒孢病菌在侵染过程中的互作关系，明确了其在病原菌接种不同时间段的差异表达特征。本研究为橡胶树棒孢霉落叶病抗病性早期鉴选、抗病种质培育与创制利用提供了很好的种质材料和理论支撑。更多还原

【Abstract】 Corynespora leaf fall disease is one of the most severe leaf diseases in major rubber planting countries worldwide, which can cause serious yield and economic losses. The selection, creation, and utilization of resistant germplasms are the most effective prevention and control strategies for this disease. This study evaluated the disease resistance of 821 F₁ populations from three hybrid combinations Yunyan 277-5×IAN 873, RRIC103×Reyan 8-79, Yunyan 277-5×Reken 525 and identified the level of disease resistance. Based on the evaluation results of disease resistance,32 candidate F₁ generation individual plants were selected from two hybrid combinations that conform to normal distribution. Using three divergent clusters of Corynespora cassiicola and two evaluation methods to identify the resistant candidate F₁ clone seedlings, five resistant germplasms were ultimately obtained by measuring the activity of defense enzymes and analyzing the expression characteristics of disease-related genes in five new resistant germplasms. Further investigation confirmed that the interaction relationship between five new resistant germplasms and multi host C. cassiicola during the infection process. This study provides excellent germplasm materials and a theoretical support for the early selection, cultivation, and utilization of resistant germplasms for the disease resistance of Corynespora leaf fall disease.更多还原