【摘要】 目的 了解2018-2019年贵州省急性弛缓性麻痹（AFP）病例中非脊髓灰质炎（简称脊灰）肠道病毒（NPEV)）病原谱分子生物学特征，加强贵州省AFP监测系统的敏感性。方法 采集贵州省2018-2019年报告的AFP病例，含AFP密切接触者粪便标本，使用人横纹肌肉瘤细胞（RD）和转人脊髓灰质炎病毒受体基因的鼠肺细胞（L20B）对粪便标本进行病毒分离，对分离到的NPEV阳性标本，采用病毒蛋白（VP）1区核苷酸序列进行鉴定分型。结果 420例AFP病例中共检测到30株NPEV,10例AFP密切接触者中检测到1株NPEV;AFP病例中男273例，女147例；<1、1～<5、5～<9、9～<15岁AFP病例分别为3、125、129、163例；2-4月、5-7月、8-10月、11-1月AFP病例分别为81、100、131、108例；病例数排前三位的市州为毕节市90例、黔西南州76例、贵阳市和黔南州均为54例。31株NPEV共鉴定出16个血清型，其中，人类肠道病毒（HEV）A组有5个血清型共10株（32.26%），以柯萨奇A组病毒（CV-A）4型为主(16.13%);HEV-B组有11个血清型共21株（67.74%），以埃可病毒（ECHO）11型为主（19.35%）。HEV-A组毒株处于5个分支，且每个分支间的距离相对较远，5株CV-A4聚集为一簇；HEV-B组毒株处于2个大分支，其中5株处于一个分支，除JH2018F0102-1、JH2018F0106-1这2株遗传距离较近外，其余遗传距离相对较远；另外16株为另一个分支，且以该分支为主要流行分支，但其处于不同的小分支，且存在一定的遗传距离。结论 贵州省2018-2019年AFP病例NPEV血清型别呈多样性在传播流行，均属于HEV-A与HEV-B组，且以HEV-B组为主，需继续做好AFP病例监测。更多还原

【Abstract】 Objective To understand the pathogenic information of non-poliomyelitis enterovirus(NPEV)in acute flaccid paralysis(AFP)cases in Guizhou province,China from 2018 to 2019,and strengthen the sensitivity of AFP surveillance system in Guizhou province. Methods AFP cases reported in Guizhou province from 2018 to 2019 were collected,including fecal specimens from close contacts of AFP. Rhabdomyosarcoma(RD)cells and mouse L cells expressing the human poliovirus receptor(L20B)cells were used to isolate viruses from fecal samples,and the isolated NPEV positive specimens were identified and typed by virus protein(VP)1 region nucleotide sequence. Results A total of 30 strains of NPEV were detected in 420 AFP cases and 1 strain of NPEV was detected in 10 close contacts with AFP. There were 273males and 147 females AFP cases. There were 3,125,129,163 AFP cases in <1,1-<5,5-<9,9-<15 years old,respectively. From February to April,from May to July,from August to October,and from November to January,there were81,100,131 and 108 AFP cases,respectively. The top three cities and prefectures with the highest number of cases were Bijie city with 90 cases,Qianxinan prefecture with 76 cases,Guiyang city and Qiannan prefecture with 54 cases respectively. A total of 16 serotypes of 31 NPEVs were identified,including human enterovirus(HEV)group A,10 isolates(32.26%)from 5 serotypes,coxsackievirus A(CV-A)4 as the dominant serotypes(16.13%). HEV-B group,21 isolates(67.74%)from 11 serotypes,dominated by Echovirus(ECHO)11(19.35%). HEV-A isolates in 5 branches,relatively distant from each branch,and 5 CV-A4 isolates gathered in a cluster. The isolates of HEV-B group were in two large branches,of which 5 isolates were in one branch. The genetic distance of HEV-B group was relatively distant except for JH2018F0102-1 and JH2018F0106-1. The other 16 strains were another clade,and this clade was the main prevalent clade,but they were in different subclades,and there was a certain genetic distance. Conclusions The NPEV serotypes of AFP cases in Guizhou province from 2018 to 2019 were diverse,all belong to HEV-A and HEV-B groups,and mainly HEV-B group. AFP monitoring should be continued.更多还原