【摘要】 目的 探讨甲状腺转录因子-1(TTF-1)、天冬氨酸肽酶A(NapsinA)蛋白表达与非小细胞肺癌（NSCLC）患者临床病理特征及预后的关系。方法 收集淮南朝阳医院2014年5月-2017年8月期间诊治的70例NSCLC患者NSCLC组织及癌旁组织，根据5年随访期间患者是否死亡将其分为死亡组（n=38）和生存组（n=32）。采用免疫组化法检测组织TTF-1、NapsinA蛋白表达；采用Kaplan-Meier法绘制不同TTF-1、NapsinA蛋白表达NSCLC患者的生存曲线；采用多因素Cox回归分析NSCLC患者5年预后的影响因素。结果 NSCLC组织中TTF-1阳性率（65.71%）、NapsinA阳性率（71.43%）显著高于癌旁组织（11.43%、14.29%），差异均有统计学意义（χ~2=43.531、46.667,P均<0.05）。TTF-1、NapsinA蛋白表达与临床分期、病理类型、淋巴结转移、分化程度有关，差异均有统计学意义（χ~2=21.043、18.641、13.635、21.652,19.330、7.292、9.307、8.217,P均<0.05）；而与年龄、性别、吸烟、肿瘤直径无关，差异均无统计学意义（P均>0.05）。TTF-1、NapsinA蛋白阳性表达患者5年生存率（30.43%、32.00%）显著低于TTF-1、NapsinA蛋白阴性表达患者（75.00%、80.00%），差异均有统计学意义（χ~2=12.622、13.263,P均<0.05）。死亡组临床分期III+IV期、有淋巴结转移患者比例高于生存组，差异均有统计学意义（χ~2=4.153、16.234,P均<0.05）。TTF-1、NapsinA是NSCLC患者5年内死亡的独立危险因素（HR=2.842、2.646,P均<0.05）。结论 NSCLC组织中TTF-1、NapsinA均高表达，二者均与临床分期、病理类型、淋巴结转移、分化程度及预后有关。更多还原

【Abstract】 Objective To investigate the relationship between thyroid transcription factor-1(TTF-1)and NapsinA protein expression and clinicopathological characteristics and prognosis of patients with non-small cell lung cancer(NSCLC).Methods The NSCLC tissues and adjacent tissues of 70 NSCLC patients diagnosed and treated in the Huainan Chaoyang Hospital from May 2014 to August 2017 were collected;according to whether the patients died or not during the 5-year follow-up,they were grouped into death group of(n=38)and survival group(n=32). The expression of TTF-1 and NapsinA proteins was detected by immunohistochemistry;the survival curves of NSCLC patients with different TTF-1 and NapsinA protein expressions were drawn by Kaplan-Meier method;multivariate Cox regression analysis was applied to analyze the influencing factors of 5-year prognosis of NSCLC patients. Results The positive rates of TTF-1(65.71%)and NapsinA(71.43%)in NSCLC tissues were obviously higher than those in adjacent tissues(11.43%,14.29%),the differences were statistially significant（χ~2=43.531,46.667;P all<0.05）. The expression of TTF-1 and NapsinA proteins was related to clinical stage,pathological type,lymph node metastasis and degree of differentiation,the differences were statistially significant（χ~2=21.043,18.641,13.635,21.652;19.330,7.292,9.307,8.217;P all<0.05）,but not related to age,gender,smoking and tumor diameter(P all>0.05). The 5-year survival rate of patients with positive expression of TTF-1 and NapsinA proteins(30.43%,32.00%)was obviously lower than that of patients with negative expression of TTF-1 and NapsinA proteins(75.00%,80.00%),the differences were statistially significant（χ~2=12.622,13.263;P all<0.05）. The proportions of patients with clinical stage Ⅲ+Ⅳ and lymph node metastasis in the death group were higher than those in the survival group,the differences were statistially significant （χ~2=4.153,16.234;P all<0.05）. TTF-1 and NapsinA were independent risk factors for 5-year death in NSCLC patients(HR=2.842,2.646;P all<0.05). Conclusion TTF-1 and NapsinA were highly expressed in NSCLC tissues,and both related to clinical stage,pathological type,lymph node metastasis,degree of differentiation and prognosis.更多还原