【摘要】 微拟球藻(Nannochloropsis oceanica)生长速度快、油脂含量高，已成为生产生物柴油的理想材料。为获得中性脂产率高的藻株，本研究从染色时间和染色浓度两个方面优化了尼罗红染色测定中性脂含量的方法，对不同育种方法得到的75株微拟球藻藻株的产油能力进行了评价。采用优化后的染色条件(尼罗红浓度1 mg/mL,染色时间10 min),测定了第9天微藻的中性脂相对含量及对应的生物量，获得藻株中性脂的相对产率。研究表明，与原始藻株相比，088号转基因藻株相对产率为252.16%,甲基磺酸乙酯(EMS)诱变藻株101号相对产率为259.02%,亚硝基胍(NTG)诱变藻株231号相对产率为323.84%。本研究获得了3株中性脂产率高的微拟球藻藻株，这3株藻株有望应用于生物柴油的生产，研究结果可为高油脂含量微藻的快速筛选提供参考。更多还原

【Abstract】 Nannochloropsis oceanica has become an ideal material for biodiesel production due to its fast growth rate and high lipid content. In order to obtain high neutral lipid yielding strains, we optimized the Nile red staining method in staining time and dye concentration for determining the neutral lipid content, and evaluating the lipid producing ability of 75 strains of N. oceanica obtained with different breeding methods. Under the optimized staining conditions(Nile red, 1 mg/mL; staining time, 10 min), we assayed the relative content of neutral lipid and biomass growth on day 9 and calculated the relative yield of neutral lipids. We found that the relative yield of a transgenic strain, 088, was 252.16% of the original strain, the relative yield of an ethyl methane sulfonate(EMS) mutant, 101, was 259.02% of the original, and the relative yield of a N-methyl-N′-nitro-N-nitrosoguanidine(NTG) mutant, 231, was 323.84% of the original. Three strains with high neutral lipid yields wereapplicable for producing biofuel. Our findings provided a reference for the rapid screening of microalgal strains with high lipid content.更多还原