【摘要】 辣椒(Capsicum annuum)是茄科辣椒属一年生或有限多年生草本植物。DNA损伤结合蛋白1(damaged DNA binding protein 1, DDB1)基因在番茄(Solanum lycopersicum)和拟南芥(Arabidopsis thaliana)等模式植物中已有一定研究，但该基因在辣椒中的功能尚不明确。本研究在辣椒中克隆了番茄DDB1同源基因，命名为CaDDB1(GenBank No. XM＿016701551)。采用病毒诱导基因沉默(virus-induced gene silencing, VIGS)技术获得了辣椒CaDDB1基因沉默植株，通过观察其表型，初步鉴定了CaDDB1的生物学功能。研究结果表明，CaDDB1基因沉默后辣椒植株极显著矮化(P<0.01)，第3～6片真叶花青素含量极显著升高(P<0.01)，说明CaDDB1基因影响辣椒的生长发育并在花青素合成方面起负调控作用。本研究结果为辣椒生长发育调控及品种改良提供一定的理论指导。更多还原

【Abstract】 Pepper(Capsicum annuum) is an annual or limited perennial herb of the Solanaceae and Capsicum genus. The damaged DNA binding protein 1(DDB1) gene has been reported in tomato(Solanum lycopersicum),Arabidopsis thaliana and other model plants, but its function in pepper is still unclear. In this study, tomato DDB1 homologous gene was cloned from pepper and named CaDDB1(GenBank No. XM＿016701551).CaDDB1 gene silencing plants were obtained by using virus-induced gene silencing(VIGS) technique, and the biological functions of CaDDB1 were preliminarily identified through observing their phenotypes. The results showed that pepper plants were extremely significantly dwarfed after CaDDB1 gene silencing(P<0.01).Simultaneously, anthocyanin content in the 3rd to 6th true leaves were extremely significantly increased(P<0.01), indicated that CaDDB1 gene affected the growth and development of pepper and played a negative role in the regulation of anthocyanin synthesis. The results of this study provide some theoretical guidance for the growth and development regulation and variety improvement of pepper.更多还原