【摘要】 目的:探讨马钱苷对严重烧伤大鼠肠道屏障的保护作用及分子机制。方法:将30只雄性SD大鼠随机分为对照组、烧伤组与马钱苷组，每组各10只。将烧伤组及马钱苷组大鼠背部造成30%TBSA III度烧伤，将对照组大鼠模拟致假烧伤。马钱苷组大鼠予以5 mg马钱苷灌胃，对照组、烧伤组均予以等量生理盐水灌胃，1次/d,持续7 d。给药结束后，采集大鼠门静脉血，采用异硫氰酸荧光素(FITC)-葡萄糖荧光探诊示踪法检测肠道通透性；采用蛋白印迹法检测大鼠肠黏膜组织白细胞介素-1β(IL-1β)、IL-6蛋白表达水平，并检测大鼠回肠组织沉默调节蛋白1(SIRT1)、核转录因子-κB(NF-κB)p65蛋白表达水平。结果:与对照组比较，烧伤组、马钱苷组肠道通透性均增高(P<0.05),但马钱苷组低于烧伤组(P<0.05);烧伤组、马钱苷组肠黏膜IL-1β、IL-6水平增高(P<0.05),但马钱苷组肠低于烧伤组(P<0.05)。与对照组比较，烧伤组和马钱苷组回肠组织SIRT1表达水平下降(P<0.05),NF-κB p65表达水平增高(P<0.05),但与烧伤组比较，马钱苷组回肠组织SIRT1表达水平增高(P<0.05),NF-κB p65表达水平降低(P<0.05)。结论:马钱苷对严重烧伤大鼠肠道屏障具有保护作用，其机制可能与介导SIRT1/NF-κB信号通路、抑制炎症反应有关。更多还原

【Abstract】 Objective:To investigate the protective effect of loganin on intestinal barrier in severely burned rats and explore the possible molecular mechanism.Methods:30 male SD rats were randomly divided into control group, burn group and loganin group, with 10 rats in each group.The rats in the burn group and loganin group were subjected to 30 % TBSA III degree burn on the back, and the rats in the control group were subjected to sham burn.The rats in the loganin group were intragastrically administered with 5 mg loganin by gavage, the other two groups were given the same amount of normal saline by gavage, once daily for 7 days.After administration, the portal vein blood of mice was collected, and the intestinal permeability was detected by fluorescein isothiocyanate(FITC)-glucose fluorescence diagnostic tracer method.Western blotting was used to detect the expression of interleukin(IL)-1β and IL-6 in rat intestinal mucosa, and the expression of silent information regulatorl(SIRT1) and nuclear transcription factor-κB(NF-κB) p65 in rat ileum tissue.Results:Compared with the control group, the intestinal permeability of burn group and loganin group increased(P<0.05),the intestinal permeability of the loganin group was lower than that of the burn group(P<0.05).Compared with the control group, the levels of IL-1β and IL-6 in intestinal mucosa of the burn group and loganin group increased(P<0.05),and the loganin group was lower than the burn group(P<0.05).Compared with the control group, the expression of SIRT1 in ileal tissues of burn group and loganin group decreased(P<0.05),and the expression of NF-κB p65 increased(P<0.05).Compared with burn group, the expression of SIRT1 in ileal tissue of loganin group increased(P<0.05),and the expression of NF-κB p65 decreased(P<0.05).Conclusion:Loganin has protective effect on intestinal barrier in severely burned rats, and its mechanism may be related to mediating SIRT1/NF-κB signaling pathway and inhibiting inflammatory response.更多还原