【摘要】 目的:探讨SCD1在TFE3-rRCC中的表达及对TFE3-rRCC细胞增殖和迁移的影响。方法:通过GEPIA数据库分析SCD1在不同肿瘤中的表达水平以及对患者预后的影响。通过QPCR和Western印迹检测SCD1在TFE3-rRCC患者和细胞系UOK109、UOK120的表达水平。利用shRNA敲低细胞系中SCD1的表达。通过CCK-8、Transwell等实验，检测细胞在敲低SCD1前后的增殖、迁移能力的变化。结果:SCD1在3种常见肾癌中的表达水平均较高，且SCD1高表达的患者生存期更短，预后更差(Logrank P<0.001)。在TFE3高度表达患者的肾癌组织以及TFE3-rRCC细胞系UOK109、UOK120中，SCD1在mRNA和蛋白水平也均显著提高。敲低SCD1后，UOK109和UOK120细胞的增殖和迁移能力均明显下降。结论:SCD1在TFE3-rRCC中高度表达，并且能够促进TFE3-rRCC细胞系的增殖和迁移能力，可能是促进TFE3-rRCC发生发展的关键分子。更多还原

【Abstract】 Objective: To investigate the expression of SCD1 in TFE3-rRCC and its effect on the proliferation and migration of TFE3-rRCC cells. Methods: GEPIA database was used to analyze the expression level of SCD1 in different tumors and its effect on the prognosis of patients. The expression levels of SCD1 in TFE3-rRCC patients and cell lines UOK109 and UOK120 were detected by QPCR and Western blot. Liposomal shRNA was used to knock down SCD1 expression in cell lines. The changes of cell proliferation and migration ability before and after SCD1 knockdown were detected by CCK-8 and Transwell experiments. Results: SCD1 expression levels were higher in all three common renal cancers, and patients with high SCD1 expression had shorter survival and worse prognosis(Logrank P<0.001). The mRNA and protein levels of SCD1 were also significantly increased in renal cancer tissues of patients with high expression of TFE3 and in TFE3-rRCC cell lines UOK109 and UOK120. After SCD1 knockdown, the proliferation and migration ability of UOK109 and UOK120 cells decreased significantly. Conclusion: SCD1 is highly expressed in TFE3-rRCC and can promote the proliferation and migration of TFE3-rRCC cell lines, which may be a key molecule in promoting the development of TFE3-rRCC.更多还原