【摘要】 偏穗鹅观草（Roegneria komarovii Nevski）是小麦族鹅观草属的植物，具有抗旱、抗病等特点，是多年生优良牧草，也是小麦三级资源库。为分析偏穗鹅观草耐盐机制及抗病基因的表达，筛选出偏穗鹅观草在不同非生物胁迫条件下稳定表达的内参基因，以偏穗鹅观草根、茎、叶对照组及盐胁迫处理组的偏穗鹅观草为材料，利用实时荧光定量PCR (qRT-PCR)技术，对肌动蛋白（Actin1、Actin2、Actin3）、微管蛋白（BUTB）、亲环素（Cyclophilin）、甘油醛-3-磷酸脱氢酶（glyceraldehyde-3-phosphate,GAPDH）、组蛋白（histone 3, H3）、延伸因子（EF1a1）8个常用小麦内参基因进行筛选；并借助geNorm、BestKeeper软件对内参基因表达的稳定性进行评价，进一步利用SnRK2基因对获得的内参基因的稳定性和可靠性进行验证。结果表明，2个软件筛选出的内参基因基本一致，8个候选内参基因在偏穗鹅观草不同胁迫处理下的表达丰度及稳定性存在差异，盐处理及干旱处理下Cyclophilin、GAPDH稳定性表现最好，高温处理下EF1a1、Cyclophilin稳定性最好。综合分析表明，Cyclophilin、GAPDH及EF1a1在偏穗鹅观草中表达丰度相对较高，并且在器官中表达较为稳定。通过对盐、高温及干旱处理组的稳定性评价，确定Cyclophilin为偏穗鹅观草最适内参基因。研究结果为后续探讨偏穗鹅观草抗逆性分子机制奠定了基础。更多还原

【Abstract】 Roegneria komarovii is a plant of Roegneria spp in Triticeae with the characteristics of drought and disease resistance, and it also is an excellent perennial forage. In order to analyze the mechanism of salt tolerance and the expression of disease resistance genes by real-time quantitative PCR(qRT-PCR), the reference genes that expressed stably under different abiotic stress were screened. The expression of 8 wheat internal reference genes were assessed including Actin protein(Actin1, Actin2, Actin3), beta-tubulin(BTUB), Cyclophilin, glyceraldehyde-3-phosphate(GAPDH), histone 3(H3) and elongation factor-1-alpha-subunit(EF1a1) in Roegneria komarovii samples under the stresses of salt, drought and high temperature, and using geNorm and BestKeeper softwares to evaluate the stability of them. SnRK2 gene was used to verify the stability and reliability of the selected internal reference genes.Based on the statistical analysis of qRT-PCR results,the expression abundance and stability of 8 candidate reference genes were different under different stress treatments. The stability of Cyclophilin and GAPDH were the best under salt and drought treatments. EF1a1 and Cyclophilin had the best stability under high temperature treatment. The results showed that Cyclophilin, GAPDH and EF1a1 were highly expressed and stable in the various organs.Cyclophilin was selected as the most suitable reference gene through the stability evaluation of abiotic stress. The results provided foundation for investigating the molecular mechanism of salt stress tolerance in Roegneria komarovii.更多还原