【摘要】 硒（Se）能抑制水稻对镉（Cd）和砷（As）的吸收，但针对Se应用风险的研究还较为缺乏。为探究Se在降低重（类）金属吸收实际应用过程中尤其是南方多雨条件下的适用性，本研究采用全生育期淹水的农艺措施，利用根施海泡石30 mg·kg^-1（SP30）和50mg·kg^-1（SP50），并配合叶面喷施Se肥（Na₂SeO₃）1 mg·L^-1（Se1）和5 mg·L^-1（Se5），测定根际土壤pH和Eh，水稻籽粒Cd、As、Se、氨基酸的含量以及Se形态等指标，探究Se对水稻生长和重（类）金属吸收的影响。结果表明：与CK处理相比，单独叶面喷施Se和单独根施海泡石处理均在一定程度上对水稻产生了不利影响，同时还一定程度上促进了籽粒中Cd或As的富集。与SP30处理相比，额外添加Se显著增加了株高（Se5SP30处理）、200粒质量（Se5SP30处理）、结实率、籽粒中Cd的含量和部分氨基酸含量，但降低了籽粒中Mg（Se5SP30处理）、Ca、Mn（Se5SP30处理）和Fe的含量。与SP50处理相比，额外添加Se显著降低了茎鲜质量、产量（Se5SP50处理）、籽粒中Mg（Se5SP50处理）、Ca（Se5SP50处理）、Fe、Zn和缬氨酸（VAL）（Se1SP50处理）的含量；增加了结实率（Se5SP50处理）和籽粒中精氨酸、色氨酸的含量，未显著影响籽粒中As和Cd含量。研究表明，全生育期淹水条件，施用Se和海泡石会增加As和Cd在水稻籽粒中富集的风险。因此，在利用Se和海泡石降低水稻籽粒中的As、Cd含量时，需要注意土壤水分的管理。更多还原

【Abstract】 Selenium（Se） can inhibit the absorption of cadmium（Cd） and arsenic（As） in rice, and combined Se and passivator technologies have been applied in rice soil remediation. However, the risks of Se application with passivators have not been investigated.Using a full flooding condition, this study explored the applicability and risks of Se combined with passivators under rainy weather in the south of China. Either 30 mg·kg^-1or 50 mg·kg^-1sepiolite（SP30 and SP50, respectively）was used as a base fertilizer and 1 mg·L^-1or 5mg·L^-1Se（Na₂SeO₃; Se1 and Se5, respectively）as a foliar fertilizer. Indexes were measured for soil rhizosphere（pH and Eh）and rice grain（mainly concentrations of Se speciation, amino acids, cadmium, arsenic, and selenium）. The results showed that compared to the CK treatment, adding Se or sepiolite alone had adverse effects on rice and promoted the enrichment of Cd or As in grains. Compared to treatment with SP30, Se1SP30 and Se5SP30 significantly increased seed setting rate and Cd concentration. Se5SP30 also significantly increased plant height and 200 grain weight. While the rice grain concentration of some amino acids increased with both treatments, a decrease in Ca and Fe was observed with both treatments, and Mg and Mn decreased in Se5SP30. Compared to treatment with SP50,Se1SP50 and Se5SP50 significantly reduced the shoot fresh weight, while treatment with Se5SP50 also reduced shoot yield and increased seed setting rate. Decreased grain concentrations of Fe and Zn were observed for both treatments, Mg and Ca with Se5SP50, and valine with Se1SP50. Increased grain concentrations of arginine and tryptophan were observed with both treatments. The concentrations of As and Cd in grains were not significantly affected with either treatment. The results of this study indicate that the use of Se or sepiolite increase the risk of As and Cd enrichment in rice grains under flooding conditions during the whole growth period. In practice, attention to water management is needed when using Se and sepiolite to remediate As or Cd contaminated soils.更多还原