【摘要】 目的 探究石墨烯材料对免疫抑制小鼠免疫功能的调节作用。方法 本研究将环磷酰胺（CTX）诱导的免疫抑制小鼠分为5组，分别模型组（CTX）、碳纤维低温度组（CF-Low,30℃）、碳纤维高温度组（CF-High,35℃）、石墨烯低温度组（G-Low,30℃）、石墨烯高温度组（G-High,35℃），另设空白对照组（CTL）。石墨烯照射组于石墨烯仓中照射，每2天用石墨烯仓照射1次，每次1 h，连续14 d；同时，另外2组于碳纤维材料仓中照射，作为等温对照；末次照射后剖检计算小鼠脾脏和胸腺指数、用血细胞计数仪检测白细胞的数量、采用MTT法检测脾脏淋巴细胞增殖的能力、流式细胞术检测血液中CD4+T/CD8+T比值、LDH法检测脾脏NK细胞杀伤活性。结果 照射7 d或14 d后，与CTX组比较，CF-Low小鼠上述指标均未见显著变化（P>0.05）；照射7 d后，G-High小鼠淋巴细胞增殖能力比CTX组显著提高（P<0.05），其他指标未见明显变化；照射14 d后，G-High小鼠胸腺指数、脾脏指数、白细胞数量、CD4+T/CD8+T比值比CTX组显著升高（P<0.05）,NK细胞杀伤活性、淋巴细胞增殖能力比CTX组显著增强（P<0.05）。而作为等温对照CF-High照射7 d后，免疫抑制小鼠淋巴细胞增殖能力比CTX组显著提高（P<0.05）；照射14 d后，只观察到淋巴细胞增殖能力及CD4+T/CD8+T比值比CTX组显著升高（P<0.05），且该组出现小鼠死亡的情况。结论 石墨烯材料对CTX诱导的小鼠免疫抑制具有一定程度的修复作用，且与石墨烯的作用温度正相关，这将拓展石墨烯在医药方面的应用。更多还原

【Abstract】 This study was designed to explore the regulatory effect of graphene materials on the immune function of immunosuppressed mice. The immunosuppressed mice were induced by cyclophosphamide(CTX) and divided into 5 groups: model group(CTX), carbon fiber low temperature group(CF low), carbon fiber high temperature group(CF high), graphene low temperature group(G-low) and graphene high temperature group(Ghigh). Normal mice was recruited as blank control group(CTL). Mice in G groups were irradiated in the graphene chamber, while the other two CF groups were used carbon fiber material as the isothermal controls; After the last irradiation, the spleen and thymus indexes of mice were calculated by dissection, the number of white blood cells was detected by blood cell counter, the proliferation ability of spleen lymphocytes was detected by MTT method, the CD4~+T/CD8~+T ratio in blood was detected by flow cytometry, and the killing activity of spleen NK cells was detected by LDH method. Data showed that after 7 days of irradiation, the lymphocyte proliferation ability of Ghigh mice was significantly improved compared with that of CTX group(P<0.05), and other indexes had no significant changes; After 14 days of irradiation, the thymus index, spleen index, white blood cell number, CD4~+T/CD8~+T ratio of G-high mice were significantly higherthan those of model mice(P<0.05), and NK cell killing activity and lymphocyte proliferation ability weresignificantly enhanced(P<0.05). In conclusion, graphene materials have a certain degree of repair effect on CTX-induced immunosuppression in mice, which is positively related to the action temperature of grapheme, and willexpand the application of graphene in medicine.更多还原