【摘要】 目的 观察OK-432(主要活性成分为OK-PSA)体外对人卵巢癌细胞株SKOV3凋亡的影响，探讨其作用机制。方法 对数生长期的SKOV3细胞随机分为对照组和OK-432给药组(浓度分别为10、20、40、80、160mg/L),给药24h之后，倒置显微镜观察细胞形态，MTT法测定OK-432对SKOV3细胞增殖的影响。应用20mg/L的OK-432处理SKOV3细胞，免疫荧光法检测内质网功能相关蛋白PDI随时间表达情况，Western blotting检测SKOV3肿瘤细胞内质网应激和凋亡相关蛋白随时间表达水平。结果 MTT法检测结果显示，与对照组相比较，OK-432可呈剂量依赖性抑制SKOV3细胞增殖(P<0.05),20mg/L的OK-432即可使肿瘤细胞变圆，体积缩小，脱壁细胞增多；随着OK-432给药时间延长，PDI在细胞核周聚集的量逐渐增加，凋亡相关蛋白cleaved-caspase 3、内质网应激相关蛋白Grp-78、CHOP表达量也逐渐增加。结论 OK-432可能通过内质网应激来诱导SKOV3凋亡。更多还原

【Abstract】 Objective To observe the effect of OK-432(OK-PSA as the main active component) on apoptosis of human ovarian cancer cell line SKOV3 in vitro, and to explore its mechanism.Methods SKOV3 cells in logarithmic growth phase were randomly divided into the control group and the OK-432 treated group(concentrations of 10,20,40,80 and 160mg/L,respectively),and the cell morphology was observed by inverted microscopy after 24 hours of administration, and the effect of OK-432 on the proliferation of SKOV3 cells was determined by MTT assay.OK-432 at 20mg/L was applied to treat SKOV3 cells, and the expression of endoplasmic reticulum function-related protein PDI over time was detected by immunofluorescence, and the expression levels of endoplasmic reticulum stress and apoptosis-related proteins in SKOV3 tumor cells over time were explored by Western blotting.Results The results of MTT assay showed that OK-432 could inhibit the proliferation of SKOV3 cells in a dose-dependent manner compared with the control group(P<0.05).OK-432 at 20mg/L could not only make tumor cells round, but also reduced their size and increased the number of desquamated cells.With the prolongation of OK-432 administration time, the amount of PDI aggregation in the perinuclear cells gradually increased, and the expression of apoptosis-related proteins cleaved-caspase 3,endoplasmic reticulum stress-associated protein Grp-78 and CHOP also increased gradually.Conclusion OK-432 may induce SKOV3 apoptosis through endoplasmic reticulum stress.更多还原