【摘要】 [目的]揭示九叶青花椒叶绿体基因组的结构特征及其与其他物种的系统进化关系，为花椒属种质资源的鉴定、新品种的培育及品种间的遗传分析提供参考。[方法]使用改良CTAB法提取九叶青花椒叶片总DNA。利用BGISeq-500平台进行高通量测序，SPAdes v3.13.0软件组装叶绿体基因组，通过GeSeq注释九叶青花椒的全叶绿体基因组信息，对其叶绿体基因组序列的结构特征、重复序列、密码子偏好性及系统进化关系进行分析。[结果]九叶青花椒叶绿体基因组为典型的四分体组成结构，全长序列为158 579 bp，编码133个基因；测到19个串联重复序列，49个长片段重复，70个简单重复序列；九叶青花椒叶绿体基因组中的蛋白编码基因共有26 398个密码子（不包括终止密码子），在密码子第三位碱基上有较强的A/T碱基偏好性。[结论]本研究首次组装了九叶青花椒叶绿体基因组全序列，明确了花椒属为单系类群，九叶青花椒与野花椒（Z. simulans）关系密切，这将为花椒的遗传信息、花椒种质资源评价、分子育种、cpSSR分子标记的开发及遗传多样性等研究提供了重要参考。更多还原

【Abstract】 [Objective]To reveal the structural characteristics and phylogenetic relationships of the chloroplast genomes of Zanthoxylum armatum ’Jiuyeqing’, and provide reference for identifying germplasm resources, breeding new varieties and the genetic analysis among varieties of the genus Zanthoxylum.[Method]The total DNA of Z. armatum ’Jiuyeqing’ was extracted using a modified CTAB method. Highthroughput sequencing was performed using the BGISeq-500 platform, the chloroplast genome was assembled by SPAdes v3.13.0 software, the whole chloroplast genome information of Z. armatum ’Jiuyeqing’was annotated by GeSeq software, and the structural characteristics, repetitive sequences, codon preference and phylogenetic relationship were analyzed. [Result]Z. armatum ’Jiuyeqing’ was a typical tetrad structure with a full-length sequence of 158 579 bp, encoding 133 genes. 19 tandem repeats were detected, 49 long repeat sequences, 70 simple sequence repeat(SSR) were detected. A total of 26 398 codons(excluding stop codons) were detected in the protein-coding gene of the chloroplast genome of Z. armatum’Jiuyeqing’, and there was a strong A/T base preference at the third base of the codon. [Conclusion]The complete chloroplast genome sequences of Z. armatum ’Jiuyeqing’ was firstly assembled for the study.The results of phylogenetic analysis show that the genus Zanthoxylum is a monophyletic group, and the Z.armatum ’Jiuyeqing’ is closely related to Z. simulans. The results of the study enrich the genetic information of Zanthoxylum and improve important references for Zanthoxylum germplasm resource evaluation,molecular breeding, development of cpSSR molecular markers, and genetic diversity research.更多还原