【摘要】 [目的]初步探究无瓣海桑的耐盐分子机制，筛选出无瓣海桑抗盐候选基因，为后续功能验证实验及林木抗盐性遗传育种奠定分子基础。[方法]以1年生无瓣海桑幼苗为材料，用500 mmol·L^-1 NaCl分别处理0 d（对照组）和10 d（处理组），取不同条件下的根部组织进行转录组测序，并结合三代全长转录组数据进行后续生物信息学分析。[结果]（1）与对照组相比，NaCl处理10 d后，无瓣海桑幼苗根系中共有14 401个差异表达基因，其中，7 153个上调，7 248个下调。（2）GO分析发现，共有11 068个差异基因在47个GO条目得到注释。（3）在KEGG富集分析中，共有6 189个差异基因富集到134条通路，其中，共有14条通路显著富集（P值<0.01,Q值<0.05）。（4）通过进一步对差异基因进行功能注释分析，共筛选出抗盐候选基因89个，其中，抗氧化基因24个，渗透调节物质基因22个，植物激素基因19个，蛋白激酶基因10个，转录因子基因14个。[结论]活性氧清除、渗透调节、植物激素、蛋白激酶及转录因子相关基因参与调控无瓣海桑盐逆境适应过程。更多还原

【Abstract】 [Objective]To lay a molecular basis for functional verification experiment and genetic breeding of tree salinity tolerance, the genetic mechanisms was explored, and salt-related genes were identified for Sonneratia apetala Buch.-Ham. [Method]In this study, the root tissues were collected from a 1-year-old S.apetala treated with 500 mmol·L^-1 NaCl for 0（control group） and 10 d（treatment group）. Then transcriptome sequencing and bioinformatics analysis was performed based on the three-generation full-length transcriptome dataset of S. apetala. [Result]（1） Compared with the control group, 14 401 genes were differentially expressed after salt treatment, of which 7 153 were up-regulated and 7 248 were down-regulated.（2） GO analysis found that a total of 11 068 differential genes were annotated in 47 GO items.（3） For KEGG enrichment analysis, a total of 6 189 differential expression genes were enriched to 134 pathways,of which 14 were significantly enriched（P-value <0.01, Q-value <0.05）.（4） Further functional annotation analysis of the differentially expressed genes revealed a total of 89 genes was potential salt-related candidate genes. Among these, 24, 22, 19, 10 and 14 genes encoded enzymes or functional proteins referred to antioxidation, osmotic adjustment substances, plant hormones, protein kinase and transcription factors,respectively. [Conclusion]Genes relating to active oxygen scavenging, osmotic regulation, plant hormones, protein kinases and transcription factors participate in the regulation of salt stress adaptation in S.apetala.更多还原