【摘要】 【目的】在枫香根际接种植物促生微生物（PGPM），研究PGPM对土壤理化性质和枫香叶色的影响以及对枫香根际微生物群落组成结构和多样性特征的改变，探明外源PGPM对植物和土壤微生物的影响。【方法】以句容市句容林场长势一致的7年生枫香为对象，通过田间接种试验并利用Illumina MiSeq高通量测序技术研究接种黏质沙雷氏菌（NJ2D）、摩西斗管囊霉（BJ04）对枫香根际土壤理化性质、叶色参数及根际土壤微生物群落结构和多样性特征的影响。【结果】1）处理组的枫香根际土壤全氮、全磷、土壤有机质、有效磷含量、土壤含水率（%）和土壤pH较对照均显著提高（P <0.05）;2）单接种BJ04菌剂的枫香叶片中叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量均较对照显著提高（P <0.05），而双接种NJ2D+BJ04菌剂枫香叶片的花青素含量显著提高（P <0.05）;3）对照与处理组的枫香根际微生物多样性也存在显著差异（P <0.05），其中，单接种NJ2D、BJ04和双接种NJ2D+BJ04菌剂的枫香根际细菌的OTU（操作分类单元）数量较对照均显著增加（P <0.05），而丛枝菌根真菌（AMF）的OTU数量较对照均显著降低（P <0.05）;4）根际微生物高通量测序结果显示，放线菌门、变形菌门、绿弯菌门、酸杆菌门和芽单胞菌门为枫香根际土壤中主要优势细菌菌群；球囊霉属、根生囊霉属和Dominikia为枫香根际土壤中主要AMF的优势菌群。接种处理后，枫香根际的酸杆菌门、绿弯菌门和浮霉菌门的物种丰度较对照均显著减小（P <0.05），而变形菌门和放线菌门的物种丰度较对照则显著增加（P <0.05）。【结论】接种解磷菌、丛枝菌根真菌以及复合菌剂NJ2D+BJ04均可改善土壤微环境，并对枫香秋季叶色变化产生影响。更多还原

【Abstract】 【 Objective】 In this study, plant growth-promoting microorganisms(PGPM) were used to inoculate into the rhizosphere of Liquidambar formosana to investigate effects of PGPM on soil physicochemical properties and the leaf color of L. formosana, as well as changes in the composition and diversity characteristics of the rhizosphere microbial community, in order to study the effect of PGPM on plants and soil microorganisms. 【Method】A field inoculation experiment was conducted on7-year-old L. formosana seedlings with consistent growth in Jurong Forest Farm of Jurong City with phosphate solubilizing bacteria Serratia marcescens subsp. marcescens(NJ2D) and Funneliformis mosseae(BJ04), and the effects on soil physical and chemical properties, leaf color parameters in autumn and soil microbial community were analyzed. 【 Result】 1) The total nitrogen, total phosphorus, soil organic matter, available phosphorus content, soil moisture content(%), and soil pH of the rhizosphere soil of L. formosana in the treatment group were significantly increased compared to the control group(P<0.05).2) The content of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in leaves of L. formosana inoculated once with BJ04 significantly increased(P<0.05), while NJ2D+BJ04 inoculation significantly increased the anthocyanin level in the leaves(P<0.05). 3) There were significant differences in the rhizosphere soil microbial community diversity between control group(CK),NJ2D, BJ04 and NJ2D+BJ04 treatments(P<0.05). The number of OTUs(operating taxonomic units) of rhizosphere bacteria in L. formosana trees inoculated with NJ2D, BJ04, and NJ2D+BJ04 was significantly increased compared to the control group(P<0.05), while the number of OTUs of arbuscular mycorrhizal fungi(AMF) was significantly reduced compared to the control group(P<0.05). 4) The high-throughput sequencing results of rhizosphere microorganisms showed that Actinobacteria,Proteobacteria, Chloroflexi, Acidobacteria and Gemmatimonadetes dominated the bacterial communities on the rhizosphere of L. formosana. Within the AMF community, Glomus, Rhizophagous and Dominika were dominant genera. However, after inoculation, the species abundances of Acidobacteria, Chloroflexi and Planctomycetes were significantly reduced within the rhizosphere of L. formosana(P<0.05), whereas Proteobacteria and Actinobacteria were significantly increased(P<0.05).【Conclusion】Inoculation with phosphorus solubilizing bacteria, arbuscular mycorrhizal fungi, and composite microbial agents NJ2D+BJ04 can all influence the soil microenvironment and have an impact on the autumn colour of L. formosana leaves.更多还原