【摘要】 建立高效液相色谱（HPLC）法测定芝麻油中芝麻素和芝麻林素的方法。结果表明：方法以甲醇-水为流动相，梯度洗脱；提取溶剂为乙腈；净化剂为50 mg硅胶键合乙二胺-N-丙基（PSA）+50 mg C₁₈;芝麻素和芝麻林素在1～100μg/mL范围内呈线性关系，相关系数（r）均大于0.998;方法检出限（LOD）为0.03 mg/g、定量限（LOQ）为0.06 mg/g。加标回收率和相对标准偏差（RSD）均符合GB/T 27417—2017的要求。方法前处理操作简单、重复性好，适用于芝麻油中芝麻素和芝麻林素的测定。更多还原

【Abstract】 The method for determination of sesamin and sesamolin in sesame oil by high performance liquid chromatography（HPLC） was established. The results showed that the method used methanol-water as the mobile phase with gradient elution.Acetonitrile was used as the extraction solvent.50 mg silica gel bonded ethylenediamine-N-propyl（PSA）+50 mg C₁₈ was used as the purifying agent. Sesamin and sesamolin had good linearity in the ranges of 1～100 μg/mL, and the correlation coefficients（r） was greater than 0.998. The limit of detection（LOD） was 0.03 mg/g and the limit of quantification（LOQ） was 0.06 mg/g. The recovery rate and relative standard deviations（RSD） meet the requirements of GB/T 27417—2017. The method was simple and reproducible, and suitable for the determination of sesamin and sesamolin in sesame oil.更多还原