【摘要】 粗茎鳞毛蕨具有极高的观赏价值和生态价值。为加强蕨类开发应用，采用开放培养基即土壤培养基（草炭土、针叶土、田园土、阔叶土、黄泥土）及传统培养基（MS、1/2MS、1/4MS）对粗茎鳞毛蕨孢子进行无菌萌发培养。结果表明：草炭土培养基的孢子最早萌发，仅需6 d就萌发，萌发率最高81.43%，培养12 d达到片状体阶段。添加蔗糖5 g·L^-1的1/2MS培养基孢子15 d萌发率最高88.93%。该研究为实现粗茎鳞毛蕨规模化无菌生产奠定了基础。更多还原

【Abstract】 Dryopteris crassirhizoma has extremely high ornamental and ecological value. In order to strengthen the development and application of ferns and enrich the species of landscape plant,the spores were cultured using open culture medium（peat soil medium,coniferous soil medium,pastoral soil medium,broad-leaved soil medium）and traditional culture medium（MS,1/2MS,1/4MS）,respectively. The results showed that the spores of the peat soil medium germinated earliest,taking 6 days,with a maximum germination rate of 81.43% and reaching the stage of flaky bodies.The highest spore germination rate was 88.93% when sucrose 5 g·L^-1 was added to 1/2MS medium. This study lays the foundation for achieving large-scale aseptic production of Dryopteris crassirhizoma.更多还原