【摘要】 以循环肿瘤细胞(CTC)的特异性膜蛋白EpCAM为识别受体，以羧基荧光素(FAM)标记的EpCAM核酸适体(Apt-FAM)为识别配体，当Apt-FAM特异性识别EpCAM后，细胞表面分布着大量的FAM荧光团，FAM荧光团的疏水性诱导非离子型表面活性剂Tween-80的疏水端在FAM周围聚积，形成以FAM为中心的伞形纳米胶束，从而增强了细胞膜的特异性识别荧光，提高了荧光信号的信噪比；利用数字信号放大技术，将成像细胞的膜荧光信号放大，使成像细胞的轮廓完整、清晰地呈现出来，实现了单细胞特异性识别轮廓的可视化，提高了捕获CTC计数和尺度估计的准确度。更多还原

【Abstract】 The specific membrane protein EpCAM of circulating tumor cell(CTC) was used as the recognition receptor, and the carboxyfluorescein(FAM)-labeled EpCAM aptamer(Apt-FAM) was used as the recognition ligand. After Apt-FAM specifically recognized EpCAM, the fluorophore of FAM was distributed abundantly on the surface of cell. The hydrophobicity of the fluorophore of FAM induced the accumulation of the hydrophobic end of the non-ionic surfactant Tween-80 around the fluorophore to form an umbrella-shaped nanomicelle centered on FAM, which enhanced the specific recognized fluorescence on the surface of cell and improved the signal-to-noise ratio of the fluorescence signal. Using digital signal amplification technology, the membrane fluorescence signal of the imaging cells was amplified, and the contours of the imaging cells were completely and clearly displayed, which realized the visualization of the contours of single cells by specific recognition, and improved the accuracy of count and scale estimation of capturing CTC.更多还原