【摘要】 [背景]多环芳烃作为一种环境污染物，具有神经毒性，它可能通过诱导炎症引起轻度认知功能障碍（MCI）。中性粒细胞与淋巴细胞比例（NLR）是一种炎症指标，是否在多环芳烃暴露和MCI关系中发挥中介作用尚不清楚。[目的]探讨外周血NLR在焦化厂工人多环芳烃暴露与MCI关系中的中介作用。[方法]采用高效液相色谱-串联质谱联用法测定530名焦炉工人尿中的11种羟基多环芳烃（OH-PAHs），使用全自动血液分析仪进行常规血液检查并计算外周血NLR。使用二元logistic回归分析尿OH-PAHs与MCI的关系，使用多元线性回归分析尿OH-PAHs与外周血NLR的关系，使用中介效应方法分析外周血NLR在尿OH-PAHs和MCI关系中的作用。[结果]在调整混杂因素和其他OH-PAHs之后，二元logistic回归结果显示工人尿中2-羟基萘（2-OHNap）和1-羟基菲（1-OHPhe）的浓度每增加e倍（e为自然对数的底数），MCI阳性的OR(95%CI)分别为1.21(1.02～1.43)和1.25(1.04～1.51);NLR浓度每增加一个单位，MCI阳性的OR(95%CI)为1.56(1.12～2.18)；多元线性回归分析结果显示焦炉工的尿1-OHPhe浓度每增加一个自然对数单位，外周血NLR水平增加0.05(95%CI:0.01～0.10)；中介分析结果显示外周血NLR在尿1-OHPhe与MCI关系中起到部分中介作用，中介比例为9.8%。[结论]焦化厂工人多环芳烃暴露可能通过外周血NLR上升而增加MCI阳性的风险。更多还原

【Abstract】 [Background] As a group of environmental pollutants, polycyclic aromatic hydrocarbons(PAHs)are neurotoxic and may cause mild cognitive impairment(MCI) by inducing inflammation.Whether neutrophil-lymphocyte ratio(NLR), an inflammatory indicator, plays a mediating role in the relationship between PAHs exposure and MCI is unclear yet.[Objective] To investigate a potential mediating role of NLR in the association between exposure to PAHs and MCI in coke oven plant workers.[Methods] Eleven urine hydroxylated PAHs(OH-PAHs) of 530 coke oven plant workers were determined by high performance liquid chromatography tandem mass spectrometry. NLR was derived from participants’ routine blood examination results using a fully automated haematology analyser. The associations between urinary OH-PAHs and MCI were analyzed by binary logistic regression, the associations between urinary OH-PAHs and NLR were analyzed by multiple linear regression, and the role of NLR in the relationship between urinary OH-PAHs and MCI was evaluated by mediating effect analysis.[Results] After controlling for confounding factors and other OH-PAHs, the results of binary logistic regression showed that for every e-fold(e is the base of the natural logarithm) increase in the concentration of 2-hydroxynaphthalene(2-OHNap) and 1-hydroxyphenanthrene(1-OHPhe), the OR(95%CI) values of reporting MCI positive were 1.21(1.02, 1.43) and 1.25(1.04, 1.51) respectively. For each unit increase of NLR, the OR(95%CI) of reporting MCI positive was 1.56(1.12,2.18). The results of multiple linear regression showed that each unit increase in natural log-trans formed levels of 1-OHPhe was associated with 0.05(95%CI: 0.01, 0.10) increase of NLR. The results of mediating effect analysis showed that the association between urinary 1-OHPhe and MCI was partially mediated by peripheral blood NLR, with a mediation ratio of 9.8%.[Conclusion] Exposure to PAHs in coke oven plant workers may increase the risk of reporting MCI positive partially through increased NLR in peripheral blood.更多还原