【摘要】 目的：建立小儿咳喘灵颗粒的薄层鉴别与含量测定的方法，加强小儿咳喘灵颗粒的质量控制。方法：采用薄层色谱法鉴别甘草、金银花、板蓝根和瓜蒌；用高效液相色谱法测定小儿咳喘灵颗粒中木犀草苷的含量，色谱柱为YMC-Pack ODS-A-C₁₈色谱柱（250 mm x 4.6 mm,5μm），流动相为乙腈和0.5%冰醋酸溶液，梯度洗脱，检测波长为350nm。结果：薄层色谱鉴别方法专属性强，斑点清晰且阴性无干扰；木犀草苷在0.95-19μg·ml^-1范围内线性关系良好，小儿咳喘灵颗粒中木犀草苷的含量为4.741μg·g^-1。结论：研究建立的TLC和HPLC均简便易行，准确可靠、重现性好，可以用于小儿咳喘灵颗粒的质量控制。更多还原

【Abstract】 Objective:The method of TLC identification and content deter mination of Xiaoer Kechuanling granules was established to strengthen the quality control of Xiaoer Kechuanling granules.Method:Radix Glycyrrhizae,Flos Lonicerae,Fructus Trichosanthis and Radix Isatidis were identified by TLC.The content of luteolin in Xiaoer Kechuanling Granules was detected by HPLC.The chromatographic column was YMC-Pack ODS-A-C₁₈ （250 mm x 4.6 mm,5μm） column.The mobile phase was acetonitrile and 0.5% glacial acetic acid solution,and the detection wavelength was 350 nm.Result:TLC identification method had strong specificity,clear spots and negative without interference.The linear range of luteolin was 0.95-～19μg·mL^-1,and the content of luteolin in Xiaoer Kechuanling Granule was 4.741μg·g^-1.Conclusion:The established TLC and HPLC are simple,accurate,reliable and reproducible,and can be used for the quality control of Xiaoer Kechuanling Granules.更多还原