【摘要】 目的 探讨LIM激酶1(LIMK1)在人肝癌细胞系Huh7中的表达及其对Huh7细胞的增殖、迁移及促进血管形成能力的影响。方法 检测人正常肝细胞系MIHA和人肝癌细胞系Huh7中LIMK1的mRNA和蛋白水平，siRNA转染Huh7细胞后通过CCK-8实验、克隆形成实验、划痕实验、Transwell实验和内皮细胞管形成实验探究对肝癌细胞增殖、迁移及血管形成能力的影响。结果 LIMK1在Huh7细胞中表达升高；敲低LIMK1后Huh7细胞的增殖及迁移能力显著下降，人脐静脉内皮细胞(HUVECs)体外血管形成能力减弱。结论 LIMK1在Huh7细胞中高表达，敲低LIMK1可抑制Huh7细胞的增殖、迁移及血管形成能力。更多还原

【Abstract】 Objective To explore the expression of LIM kinase 1(LIMK1) in human hepatocellular carcinoma cell line Huh7 and its effects on the proliferation, migration and ability to promote angiogenesis of Huh7 cells.Methods The expression of LIMK1 mRNA and protein in human normal hepatocyte line MIHA and human hepatoma cell line Huh7 was detected.After targeted siRNA transfection of Huh7 cells, the effects on the proliferation, migration and angiogenesis ability of hepatocellular carcinoma cells were investigated by CCK-8 assay, clone formation assay, wound healing assay, Transwell assay and endothelial cell tube formation assay.Results LIMK1 was highly expressed in Huh7 cells.The proliferation and migration ability of Huh7 cells were significantly decreased after knocking down the expression level of LIMK1.Human umbilical vein endothelial cells(HUVECs) have reduced in vitro angiogenic capacity.Conclusion LIMK1 was highly expressed in Huh7 cells, and knockdown of LIMK1 inhibited the proliferation, migration and ability to promote angiogenesis of Huh7 cells.更多还原