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不同外源激素处理对沙米种子萌发及胚根生长的影响

Effects of Different Exogenous Hormones on Seed Germination and Radicle Growth of Agriophyllum squarrosum

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【作者】 张德魁马全林魏林源陈芳唐卫东张卫星宋德伟胥宝一

【Author】 ZHANG De-kui;MA Quan-lin;WEI Lin-yuan;CHEN Fang;TANG Wei-dong;ZHANG Wei-xing;SONG De-wei;XU Bao-yi;State Key Laboratory of Desertification and Aeolian Sand Disaster Combating,Gansu Desert Control Research Institute;

【机构】 甘肃省治沙研究所甘肃省荒漠化与风沙灾害防治国家重点实验室培育基地

【摘要】 为研究不同外源激素对沙米种子萌发及胚根伸长生长的影响,以民勤沙区采集沙米种子为试验材料,研究不同浓度赤霉素(GA3)和氟啶酮(FL)两种外源激素及其混合叠加液对沙米种子发芽率、发芽势、发芽指数、胚根生长速度等指标的影响。结果表明:除T6和T2两个处理的发芽率小于CK(对照)外,其他处理均能有效地促进沙米种子的萌发,各处理按发芽率高低排序为T14>T9>T5>T10>T15>T1>T4>T13>T12>T11>T8>T7>T3>CK>T2>T6;FL浓度为0.1~5 mg/L,随着浓度的增加种子发芽率也在增加,浓度为10 mg/L时种子发芽率低于5 mg/L;0.5 mg/L的FL溶液浸泡处理沙米种子,浸泡时间为3~24 h,随着浸泡时间的增加,种子的发芽率在增加,但浸泡3 h(T2)破除休眠效果差,种子的发芽率低于对照,浸泡6 h以上破除休眠效果明显;GA3+FL的混合叠加液处理沙米种子,破除休眠效果最佳,叠加液处理后24 h种子的发芽率均大于50%,最高发芽率达83%,36 h发芽率均≥70%,48 h发芽率已大于80%,T14处理效果最佳,种子的发芽率达100%,发芽势达100%,发芽指数达30.86;不同处理分别在24、36、48 h时间段种子的发芽率存在较大差异,不同浓度外源激素破除沙米种子休眠促进发芽的时间不同;不同浓度外源激素处理沙米种子12 h后种子胚根均能快速伸长生长,且胚根的长度与萌发时间呈极强线性相关关系(R2>0.8),胚根长y和生长时间x可以用线性关系式y=ax+b表达。通过研究不同外源激素处理对沙米种子萌发及胚根伸长生长的影响,筛选出适宜浓度的外源激素处理方法,可为沙米的种苗培育、人工驯化栽培提供一定的理论基础及数据参考。

【Abstract】 In order to study the effects of different exogenous hormones on seed germination and radicle elongation of Agriophyllum squarrosum seeds, the Agriophyllum squarrosum seeds harvested in Minqin County were used as experimental materials to study the effects of different concentrations of gibberellin(GA3)and fluridone(FL)and their mixed superposition solutions on germination rate, germination potential, germination index, radicle growth rate and length. The results show that except that the germination rate of T6 and T2 treatments is lower than that of the CK(control), other treatments could effectively promote the germination of Agriophyllum squarrosum seeds, according to the germination rate of each treatment, the order is T14>T9>T5>T10>T15>T1>T4>T13>T12>T11>T8>T7>T3>CK>T2>T6. The germination rate of seeds increases with the concentration of FL is 0.1 to 5 mg/L,when the concentration is 10 mg/L, the germination rate of seeds is lower than that of 5 mg/L. Agriophyllum squarrosum seeds are soaked in 0.5 mg/L FL solution for 3 to 24 h, the germination rate increases with the increase of soaking time. However, the germination rate of seeds soaked for 3 h(T2)is lower than that of the CK, and the germination rate of seeds soaked for more than 6 h is obviously better than that of the CK. GA3+FL mixed superposition solution has the best effect on breaking dormancy of Agriophyllum squarrosum seeds, after the superposition solution treatment, the germination rate of the seeds is more than 50% at 24 h, the highest germination rate is 83%, the germination rate is ≥70% at 36 h, and the germination rate is more than 80% at 48 h, T14 treatment has the best effect, the germination rate is 100%, the germination potential is 100%, and the germination index is 30.86. The germination rates of different treatments are significantly different at 24, 36, 48 h, respectively. Different concentrations of exogenous hormones break the dormancy of Agriophyllum squarrosum seeds and promoted germination at different times. The radicle of Agriophyllum squarrosum seeds treat with different concentrations of exogenous hormones could elongate and grow rapidly for 12 h, and the radicle length has a strong linear correlation with germination time(R2>0.8), The radicle length y and growth time x could be expressed by a linear relationship: y=ax+b. By studying the effects of different exogenous hormone treatments on Agriophyllum squarrosum seed germination and radicle elongation growth, the appropriate concentration of exogenous hormone treatment method is selected, which can provide a certain theoretical basis and data reference for seedling cultivation and artificial domestication cultivation.

【基金】 国家自然科学基金(31560236;31270754;41361001);中央财政林业科技推广示范项目([2021]ZYTG002号);甘肃省财政林草项目(kjcx2021002);甘肃省自然科学基金(20JR10RA466);甘肃省青年科技基金(17JR5RA013)
  • 【文献出处】 科学技术与工程 ,Science Technology and Engineering , 编辑部邮箱 ,2023年20期
  • 【分类号】Q945
  • 【下载频次】26
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