【摘要】 目的 子宫内膜异位症由于其发病机制不明，至今仍是妇产科的一大难题。桂枝茯苓丸（GZFLW）是古代中药方剂之一，虽然GZFLW最初用于治疗妇科疾病，但GZFLW作用于子宫内膜异位症的分子机制尚不清楚。方法 我们分离培养原代子宫内膜异位基间质细胞（EESCs），利用GZFLW含药血清处理EESCs细胞，检测其中微小分子RNA(miRNA)的表达差异，之后利用生物信息学等手段找出GZFLW作用的miRNA及其靶基因；最后利用细胞功能实验验证GZFLW通过该途径抑制子宫内膜异位症间质细胞的迁移功能。结果 通过RNA-seq结果发现miR-23b表达下调。此外，我们利用Targetscan软件预测发现抑癌因子pten是miR-23b的下游靶基因。并且通过qPCR和Western blot验证miR-23b确实能够抑制pten转录和蛋白水平的表达。最后利用细胞迁移实验验证GZFLW-miR-23b-pten这一通路能够抑制EESCs迁移。结论 GZFLW能够通过下调miR-23b的表达，促进抑癌因子pten表达，从而抑制EESCs的迁移。更多还原

【Abstract】 Objective Endometriosis is still a difficult problem in obstetrics and gynecology because of its unknown pathogenesis. Guizhi Fuling Wan(GZFLW) is one of the ancient Chinese medicine prescriptions. Although GZFLW was originally used to treat gynecological diseases, the molecular mechanism of GZFLW’s action on endometriosis is still unclear. Methods We isolated and cultured primary ectopic endometrial stromal cells(EESCs), treated them with GZFLW drug-containing serum, and detected the expression differences of micro-RNA(miRNA) in the EESCs cells. Then bioinformatics was used to identify the mirnas directly affected by GZFLW and their target genes. Finally, cell function experiments were used to verify that GZFLW inhibited the migration function of mesenchymal cells in endometriosis through this pathway. Results The expression of miR-23b was down-regulated in RNA-seq. In addition, Targetscan software was used to predict that the tumor suppressor pten was the downstream target gene of miR-23b. Moreover, it was verified by qPCR and Western blot that miR-23b could indeed directly inhibit pten transcription and protein expression. Finally, cell migration experiments were used to verify that the GZFLW-miR-23b-pten pathway could inhibit EESCs migration. Conclusion GZFLW can inhibit the migration of EESCs by down-regulating the expression of miR-23b and promoting the expression of pten.更多还原