【摘要】 目的：研究非小细胞肺癌（NSCLC）中miR-29a-3p的表达及其对肿瘤细胞增殖和迁移的影响。方法：选取12例手术切除的NSCLC组织标本及NSCLC细胞株H1299和A549，采用实时荧光定量聚合酶链反应（RT-qPCR）检测NSCLC组织及细胞株中miR-29a-3p的表达。通过转染H1299和A549过表达miR-29a-3p，采用CCK-8和EDU实验检测miR-29a-3p过表达对NSCLC细胞增殖的影响，划痕实验检测miR-29a-3p过表达对NSCLC细胞迁移的影响。通过miRDB、RNA22、TarBase和TargetScan数据库预测miR-29a-3p靶基因，Gene Ontology(GO)富集分析探究miR-29a-3p可能的生物学通路。结果：NSCLC组织和细胞中miR-29a-3p表达水平明显降低，过表达miR-29a-3p能抑制NSCLC细胞增殖和迁移。miR-29a-3p靶基因GO富集分析显示miR-29a-3p主要与细胞外基质结构成分、内质网腔、多肽赖氨酸修饰等通路密切相关。结论：NSCLC中miR-29a-3p显著低表达，miR-29a-3p能抑制NSCLC细胞的增殖和迁移。更多还原

【Abstract】 Objective:To investigate the expression of miR-29a-3p in non-small cell lung cancer(NSCLC) and its role in the proliferation and migration of NSCLC. Methods:Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression of miR-29a-3p in NSCLC tissues, H1299 and A549 cells. NSCLC cell lines H1299 and A549 overexpressed miR-29a-3p. CCK-8 and EDU experiments were used to analyze the effect of miR-29a-3p overexpression on the proliferation of NSCLC cells, scratch assay was used to analyze the effect of miR-29a-3p overexpression on the migration of NSCLC cells. Databases of miRDB, RNA22, TarBase and TargetScan predicted the target genes of miR-29a-3p, and Gene Ontology(GO) enrichment analysis was conducted to explore the possible biological mechanism of miR-29a-3p. Results:The expression level of miR-29a-3p was significantly decreased in NSCLC tissues and cells. CCK8 and EDU experiments indicated that overexpression of miR-29a-3p could effectively inhibit the proliferation of NSCLC cells, and scratch assay indicated that overexpression of miR-29a-3p significantly inhibited the migration of NSCLC cells. GO enrichment analysis of miR-29a-3p target genes revealed that miR-29a-3p was closely related to extracellular matrix structural components, endoplasmic reticulum lumen, peptide lysine modification and other pathways.Conclusion:The expression of miR-29a-3p in NSCLC was significantly low, and miR-29a-3p can inhibit the proliferation and migration of NSCLC cells.更多还原