【摘要】 扬稻6号是杂交水稻育种中被广泛应用、具有重要地位的骨干亲本，但其稻瘟病抗性和白叶枯病抗性均普遍偏弱。通过分子标记辅助选择，将抗稻瘟病基因Pigm和Pi33、抗白叶枯病基因Xa21和Xa23导入到扬稻6号，借助重测序技术最大限度地消除每个抗性基因的连锁阻力，构建了高背景回复率近等基因系NIL^Pigm、NIL^Pi33、NIL^Xa21和NIL^Xa23,表现为稻瘟病或白叶枯病抗性的明显改良。在此基础上，进一步通过基因聚合创制了性状优良、包含所有4个抗性基因的优良株系R156,可作为水稻分子育种的核心种质。更多还原

【Abstract】 Generation of core rice germplasm with resistance to multi-diseases and great breeding potential is increasingly important in current molecular breeding program. Yangdao 6（YD6） has been widely recommended as a very important breeding parent, but it shows weak resistance to both blast and blight diseases. In this study, the elite resistance genes, Pigm and Pi33 showing broad resistance to blast, Xa21 and Xa23 displaying broad resistance to blight, were introduced into YD6 by marker assisted selection. In the aid of re-sequencing technology, the linkage drag of each resistance gene was significantly eliminated, which resulted in the development of four near isogenic lines, NIL^Pigm, NIL^Pi33, NIL^Xa21 and NIL^Xa23, in YD6 genetic background. The four NILs exhibited high background recovery rate and obvious improvement of resistance to blast and blight. Through gene pyramiding, the elite line, R156, containing all the 4 resistance genes, was developed, which showed excellent performance and could be used as a core germplasm in rice molecular breeding program.更多还原