【摘要】 目的 :提高小鼠非手术法胚胎移植成功率,改进小鼠的非手术法胚胎移植器,完善小鼠非手术法胚胎移植程序。方法 :设计3种不同的胚胎移植器,比较不同胚胎移植器的移植成功率,进一步改进移植器,优化移植程序。结果 :C型移植器的移植妊娠率和着床率(77.8%、60.3%)均高于A型(66.7%、23.0%)和B型(61.1%、45.1%)。将C型移植器移植管的长度延长至27mm,移植后的妊娠率、着床率都得到进一步提高,分别为92.9%、76.9%,出生率也达到73.1%。结论 :本研究成功改进了小鼠非手术胚胎移植器,优化了移植程序,使非手术法胚胎移植效率与手术法相当,且更加简单、快捷、易掌握。更多还原

【Abstract】 Objective : In order to increase the success rate of non-surgical embryo transfer, improve the non-surgical embryo transfer device and optimize the procedure of non-surgical embryo transfer in mice. Methods : Three different transcervical embryo transer devices were designed in this study, and the procedure of non-surgical embryo transfer in mice was optimized by comparing the success rate of different transcervical embryo transfer devices. Results : The pregnancy rate and implantation rate of type C(77.8% and 60.3%) were higher than those of type A(66.7% and 23.0%) and type B(61.1% and 45.1%). Therefore, we further improved the type C by extending the length of the tube to 27 mm, and the pregnancy rate and implantation rate after embryo transfer were further improved, which were 92.9% and 76.9% respectively, and the birth rate also reached 73.1%. Conclusion : This study has successfully improved the mice transcervical embryo transfer device, optimized the transfer procedure, made the transcervical embryo transfer as efficient as surgical embryo transfer, which is much simpler, faster, and easier to use.更多还原