【摘要】 目的 探讨pcgf5a基因在斑马鱼胚胎发育过程中的作用和机制。方法 通过显微注射pcgf5a吗啉代寡核苷酸(MO)抑制pcgf5a基因mRNA翻译过程，观察斑马鱼胚胎发育过程中相关表型的变化；利用胚胎整封原位杂交和Real-time PCR等技术检测抑制pcgf5a对胚胎神经及眼睛发育相关标记基因的表达变化。结果 抑制pcgf5a基因后，斑马鱼胚胎中外胚层发育受到影响，表现为脑区变小，眼睛发育迟缓，尾部卷曲及体轴缩短(548/891),同时神经发育(sox2:82/98、sox3:73/84、foxg1:70/88、pax6a:36/45)和眼睛发育(pax2a、vsx2和rx1,n=5)相关基因表达明显降低。结论 pcgf5a可能通过调节神经与眼睛发育相关转录因子的表达，进而影响细胞增殖和凋亡而参与胚层分化以及后续的神经系统与眼睛的发育。更多还原

【Abstract】 Objective To investigate the role of pcgf5a gene during zebrafish embryonic development. Methods pcgf5a morpholine antisense morpholino oligomers(MO) was microinjected to delete the expression of pcgf5a gene, and possible phenotypes were examined in pcgf5a-deficient embryos. The whole mount in situ hybridization and Real-time PCR were used to detect alterations of key genes related to the development of nervous system and eye. Results After pcgf5a gene knockdown, the development of ectoderm and mesoderm of zebrafish embryos were affected. The brain size became smaller, and the eyes developed retarded, and the tail was curled and the body axis was shortened(548/891). In addition, the expressions of sox2(82/98), sox3(73/84), foxg1(70/88), pax6a(36/45), pax2a, vsx2 and rx1(n=5) were significantly reduced. Conclusion pcgf5a affects the morphogenesis of nervous system and eye, possibly due to regulating the expression of the transcription factors related to their development via the processes of cell proliferation and apoptosis.更多还原