【摘要】 目的 探讨锌铁转运蛋白SLC39A6对人脑胶质瘤增殖和侵袭过程的影响。方法 选取患者来源的不同级别的脑胶质瘤组织45例及正常脑组织10例，通过实时荧光定量PCR(qRT-PCR)和Western blot方法检测组织和已有细胞系中SLC39A6的mRNA和蛋白表达水平，构建SLC39A6的RNA干扰慢病毒，利用慢病毒转染T98G和U251细胞系来敲低SLC39A6的表达，通过qRT-PCR与Western blot方法检测转染效率，MTS方法检测敲低SLC39A6后细胞的活性变化，Transwell实验检测细胞侵袭能力的变化。结果 SLC39A6 mRNA和蛋白在人脑胶质瘤组织表达水平显著高于正常脑组织，并随级别增高而增高。构建稳定敲低SLC39A6的T98G和U251细胞系，与对照组相比，敲低SLC39A6后胶质瘤细胞的增殖和侵袭能力显著降低。结论 敲低SLC39A6抑制胶质瘤细胞增殖和侵袭能力，是潜在的治疗靶点。更多还原

【Abstract】 Objective To investigate the effect of SLC39A6 on the proliferation and invasion of human gliomas.Methods The expressions of SLC39A6 mRNA and protein in forty-five glioma tissues and 10 normal brain tissues and cell lines(HA,T98G,U87,LN229,U178 and U251) were detected by qRT-PCR and Western blot.The RNA interference lentivirus of SLC39A6 was constructed and transfected into T98G and U251 cell lines to knockdown SLC39A6 expression.MTS was used to detect cell viability and Transwell assay was used to detect the change of cell invasion ability. Results The expression levels of SLC39A6 mRNA and protein were significantly higher in human glioma tissues than in normal brain tissues, and increased with the increased tumor grades.T98G and U251 cell lines with stable knockdown of SLC39A6 were constructed, the proliferation and invasion ability of T98G and U251 cells were significantly decreased in SLC39A6 knockdown than in control group. Conclusion Knockdown of SLC39A6 inhibits the proliferation and invasion of glioma cells, SLC39A6 is a potential therapeutic target of gliomas.更多还原