【摘要】 目的：探究大鼠椎间融合模型的建立以及力学测试。方法：2019年1月～2021年5月期间选取纯种健康雄性SD大鼠60只，随机分为三组，各20只，制备椎间融合模型，取双侧髂骨制成颗粒骨，A组：植入自体骨量0.3 cm~3,B组：植入自体骨量0.2 cm~3,C组：不植骨(空白对照组)。植入后分别予以放射学检查、生物力学检测及组织学观察，分析不同融合骨量对骨融合率、强度影响。结果：4 w手触检查显示：A组融合率为1只(10.00%),B组、C组未出现融合，6 w后A组融合4只(40.00%),B组融合为4只(40.00%),C组为0只(0.00%)。组织学中A组与B组融合组织中，表现为丰富骨桥，C组以大量排列紊乱胶原纤维为主，未见到新生骨组织。两组鼠骨钙素(BGP)、骨碱性磷酸酶(BALP)、转化生长因子β(TGF-β)、骨形态蛋白(BMP-2)、血管内皮生长因子VEGF)水平分别在术后0、3、5、7、14、30 d比较差异无统计学意义(P>0.05),A组、B组术后2 w、术后3 w、术后4 w X线评分高于C组，差异有统计学意义(P<0.05)。结论：本次试验模型应用手术方式和融合技术，并发症少及成功率高，与临床椎间融合相符。更多还原

【Abstract】 Objective To establish the lumbar interbody fusion models of rats and mechanical testing. Method From January 2019 to May 2021, 60 purebred healthy male SD rats were selected and randomly divided into 3 groups, with 20 rats in each group. Lumbar interbody fusion model was prepared, bilateral ilium was made into particles. Group A was implanted with 0.3 cm~3 of autogenous bone, group B with 0.2 cm~3 of autogenous bone and group C without bone graft(Blank control group). After implants, radiological examination, biomechanical detection and histological observation were performed. Impact of fusion bone mass on bone fusion rate and strength was evaluated. Results After 4w of examination, group A had one fusion case(10.00%), but without fusion cases in group B and group C; after 6w, fusion rate in group A was 40.00%(4 cases), 40.00%(4 cases) in group B and 0.00% in group C(0 case). Histological examination indicated that group A and group B had a plenty of bone bridges and group C with lots of unorderly collagenous fiber and without new bone tissues. After 0d, 3d, 5d, 7d, 14d and 30d of surgery, bone gla-protein(BGP),bone alkaline phosphatase(BALP), transforming growth factor-β(TGF-β),bone Morphogenetic protein-2(BMP-2) and vascular endothelial growth factor(VEGF) among groups were not significantly different(P>0.05); after 2w, 3w and 4w of surgery, X-ray image diagnostic scores in group A and group B were significantly higher than group C(P<0.05). Conclusion The surgical treatment and fusion techniques can reduce the complications and increase the success rate of interbody fusion.更多还原