【摘要】 目的：研究原苏木素B(PSB)对人结肠癌SW620、SW480细胞的增殖、迁移、侵袭及凋亡的影响，并探讨其抗结肠癌细胞的作用及机制。方法：实验1:将SW620、SW480细胞各分为7组，分别为对照组(不加药物经DMSO处理)和PSB-1～PSB-6组(分别给予6.25μg/ml、12.50μg/ml、25.00μg/ml、50.00μg/ml、100.00μg/ml和200.00μg/ml PSB),并分别处理24 h、48 h、72 h和96 h。实验2:将结肠癌SW620、SW480细胞各分为4组：对照组(不加药物经DMSO处理)、PSB-7～PSB-9组(分别给予17.5μg/ml、35.0μg/ml和70.0μg/ml PSB处理24 h);另外SW620细胞中又分为实验1组(25.0 ng/ml IGF-1处理24 h)、实验2组(25.0 ng/ml IGF-1+35.0μg/ml PSB处理24 h)、实验3组(1μmol/L AZD2858处理24 h)和实验4组(1μmol/L AZD2858+35.0μg/ml PSB处理24 h)。检测SW620、SW480细胞增殖、迁移、侵袭、凋亡情况、高尔基磷蛋白3(GOLPH3)及SW620细胞磷酸化蛋白激酶B(p-AKT)、磷酸化p70核糖体蛋白S6激酶(p-p70S6K)、β-连环蛋白(β-catenin)的表达水平。结果：PSB以浓度和时间依赖效应抑制SW620、SW480细胞的增殖。在SW620细胞中，PSB-8组与对照组的细胞迁移数目分别为(367.67±8.18)个和(890.00±19.65)个，细胞侵袭数目分别为(122.33±9.18)个和(374.67±13.47)个，细胞凋亡率分别为(27.74±2.57)%和(6.13±0.45)%,PSB-7～PSB-9组和对照组的GOLPH3蛋白相对表达量分别为(0.52±0.03)、(0.36±0.03)、(0.07±0.01)和(0.96±0.12),PSB-8组、对照组及实验2组中的p-AKT的蛋白相对表达量分别为(0.10±0.05)、(1.04±0.34)和(10.00±1.61),p-p70S6K的蛋白相对表达量分别为(0.40±0.14)、(1.05±0.12)和(2.52±0.29),β-catenin的蛋白相对表达量分别为(0.14±0.07)、(0.99±0.23)、(6.06±0.89),对照组、PSB-8组、实验3组和实验4组的相对荧光强度值分别为(1.00±0.07)、(0.14±0.06)、(1.98±0.16)和(1.31±0.14),差异均有统计学意义(均P<0.001);在SW480细胞中，PSB-8组与对照组的细胞迁移数目分别为(314.33±5.31)个和(589.67±22.87)个，细胞侵袭数目分别为(75.67±7.04)个和(196.33±11.90)个，细胞凋亡率分别为(22.05±0.90)%和(7.07±0.56)%,PSB-7～PSB-9组和对照组的GOLPH3蛋白相对表达量分别为(0.83±0.01)、(0.54±0.02)、(0.48±0.03)和(1.00±0.06),差异均有统计学意义(均P<0.001)。结论：PSB通过下调GOLPH3表达，抑制PI3K/AKT/mTOR和Wnt/β-catenin信号通路，从而抑制结肠癌SW620、SW480细胞的增殖、迁移、侵袭，并诱导其凋亡。更多还原

【Abstract】 Objective To study the effect of pro-hematoxylin B(PSB) on the proliferation, migration, invasion, and apoptosis of human colon cancer SW620 and SW480 cells, and to explore its anti-colon cancer cell effect and mechanism. Method Experiment 1: SW620 and SW480 cells were divided into 7 groups, namely the control group(treated with DMSO without drugs) and the PSB-1～PSB-6 groups(treated with 6.25, 12.50, 25.00, 50.00,100.00 and 200.00μg/ml PSB, respectively) and treated for 24, 48, 72, and 96 hours, respectively; Experiment 2: Colon cancer SW620 and SW480 cells were divided into four groups: control group(treated with DMSO without drugs), PSB-7-PSB-9 group(treated with 17.5, 35.0, and 70.0μg/ml PSB treatment for 24 hours, respectively); In addition, SW620 cells were divided into experimental group 1(25.0 ng/ml IGF-1 treatment for 24 hours) and experimental group 2(25.0 ng/ml IGF-1+35.0 μg/ml PSB treatment for 24 hours), experimental group 3(1 μ mol/L AZD2858 treatment for 24 hours and experimental group 4(1 μ mol/L AZD2858+35.0 μg/ml PSB treatment for 24 hours).Detection of cell proliferation, migration, invasion, apoptosis, and Golgi phosphoprotein 3(GOLPH3), phosphorylated protein kinase B(p-AKT), p70 ribosomal protein s6 kinase(p-p70S6K), β-catenin(β-catenin) expression.Results PSB inhibited the proliferation of SW620 and SW480 cells in a concentration and time-dependent manner. In SW620 cells, the number of cell migration was(367.67 ± 8.18) and(890.00 ± 19.65) in psb-8 group and control group, the number of cell invasion was(122.33 ± 9.18) and(374.67 ± 13.47), the apoptosis rate was(27.74 ± 2.57)% and(6.13 ± 0.45)% respectively, and the relative expression of GOLPH3 protein in PSB-7 ～ PSB-9 group and control group was(0.52 ± 0.03),(0.36 ± 0.03),(0.07 ± 0.01) and(0.96 ± 0.12), respectively, The relative protein expression of p-Akt in PSB-8 group, control group and experimental group 2 was(0.10 ± 0.05),(1.04 ± 0.34) and(10.00 ± 1.61) respectively, and the relative protein expression of p-p70s6k was(0.40 ± 0.14),(1.05 ± 0.12) and(2.52 ± 0.29) respectively, the relative expression levels of β-catenin protein were(0.14 ± 0.07),(0.99 ± 0.23),(6.06 ± 0.89) respectively. The relative fluorescence intensity values of control group, PSB-8 group, experimental group 3 and experimental group 4 were(1.00 ± 0.07),(0.14 ± 0.06),(1.98 ± 0.16) and(1.31 ± 0.14) respectively, the difference was statistically significant( allP<0.001);In SW480 cells, the number of cell migration was(314.33 ± 5.31) and(589.67 ± 22.87) in PSB-8 group and control group, the number of cell invasion was(75.67 ± 7.04) and(196.33 ± 11.90) respectively, and the apoptosis rate was(22.05 ± 0.90)% and(7.07 ± 0.56)% respectively. The relative expression of GOLPH3 protein in PSB-7 ～ PSB-9 group and control group was(0.83 ± 0.01),(0.54 ± 0.02),(0.48 ± 0.03) and(1.00 ± 0.06) respectively. with statistical significance(all P<0.001).Conclusion PSB inhibits the PI3K/AKT/mTOR and Wnt/β-catenin signaling pathways by downregulating the expression of GOLPH3, thereby inhibiting the proliferation, migration, and invasion of colon cancer SW620 and SW480 cells, and inducing their apoptosis.更多还原