【摘要】 以多年生黑麦草（Lolium perenne L.)幼苗为材料，用不同浓度的NaCl(A)、NaHCO3(B)、NaCl和NaHCO3混合溶液（C）进行胁迫处理，探究盐碱胁迫下黑麦草幼苗3种保护酶的分布，为研究黑麦草幼苗适应盐碱胁迫的响应机制提供理论依据。在2种单盐（NaCl、NaHCO3）处理浓度分别为100、200、300、400 mmol/L(A1、A2、A3、A4)和30、60、90、120 mmol/L(B1、B2、B3、B4),1种混合盐（NaCl+NaHCO3）处理浓度为100、200、300、400 mmol/L(C1、C2、C3、C4)，胁迫后采用聚丙烯酰胺凝胶电泳法，对黑麦草植株SOD、POD、CAT进行分离并建立电泳图谱，结果表明：3种酶在黑麦草植株内均有表达，在不同盐碱胁迫下酶谱带分布和活性均有不同，以适应盐胁迫环境下细胞内特殊的生化反应。更多还原

【Abstract】 Perennial ryegrass(Lolium perenne L.) seedlings were treated with various of concentrations of NaCl(A),NaHCO3(B), NaCl and NaHCO3(C) to investigate the distribution of three protective enzymes in Lolium perenne L.When the concentration of two kinds of single salt(NaCl, NaHCO3) were 100, 200, 300, 400 mmol/L(A1, A2, A3,A4) and 30, 60, 90, 120 mmol/L(B1, B2, B3, B4), and the concentrations of one mixed salt(NaCl + NaHCO3) were100, 200, 300, 400 mmol/L(C1, C2, C3, C4), respectively. After stressed, polyacrylamide gel electrophoresis was used to separate SOD, POD and CAT from ryegrass plants and establish electrophoresis pattern. The results showed that 3 enzymes were expressed in ryegrass plants, with variations in zymogram distribution and enzyme activity under different saline-alkali stress conditions, allowing the plants to adapt to the unique biochemical reactions occurring within cells under salt stress.更多还原