[Objective] The aim was to improve the effect of vitrification of ovine mature oocyte.[Method] With fresh mature oocytes as control,effects of cumulus cells and cytoskeleton stabilizer on vitrification of ovine mature oocyte was studied.[Result] There was no significant effect on vitrification of ovine mature oocyte with or without cumulus cells.The vitrified oocytes treated with 7.5,10.0 μg/ml CB attained a higher development rate(P<0.05,8.1 %,7.8 % respectively) to blastocyst than those of oocytes treated...