【摘要】 为开展油棕油脂代谢调控相关基因鉴定研究，以油棕八氢番茄红素脱氢酶基因（phytoene desaturase gene,PDS）作为报告基因，探索以病毒载体TRV为载体在油棕胚状体上应用病毒诱导基因沉默（virus-induced gene silencing,VIGS）的可能性，并对油棕胚状体VIGS体系的相关参数进行优化。结果显示：以EHA105为菌种、侵染菌液OD600=0.5、侵染时间5 min、乙酰丁香酮（AS）质量浓度20 mg/L、共培养48 h、侵染后培养时间为12 d能取得最佳的基因沉默效果。在此基础上，利用优化后的VIGS体系对油棕二酰甘油酰基转移酶基因（diacylglycerol acyltransferase gene,DGAT）进行沉默，取得了预期的基因沉默效果。更多还原

【Abstract】 The phytoene desaturase gene(PDS) as a reporter gene was used to study the feasibility of applying virus-induced gene silencing(VIGS) on oil palm embryoid using the TRV as a vector, and to optimize the relevant parameters. The results showed that the effect of gene silencing was the best when EHA105 was used as the strain under the condition that the OD600 of infected bacterial solution was 0.5, with infection time of 5 min, acetosyringone(AS) mass concentration of 20 mg/L, co-cultivation of 48 h, and post-infection incubation time of 12 d. On this basis, the VIGS system optimized was used to silence the diacylglycerol acyltransferase gene(DGAT), and the expected gene silencing effect was achieved.更多还原