【摘要】 目的 分析630 nm发光二极管(light emitting diode, LED)产生红光照射对人单核细胞白血病细胞(THP-1)来源M0巨噬细胞的作用与机制。方法 用佛波酯(phorbol-12-myristate-13-acetate, PMA)处理THP-1细胞，制备THP-1来源M0巨噬细胞，并使用强度为28.8 J/cm~2的630 nm LED红光照射M0巨噬细胞，随后采用非标定量蛋白质组学分析方法筛选差异表达蛋白并进行基因本体论(gene ontology, GO)注释和富集；通过string在线网站(https://cn.string-db.org/)和Cytoscape软件分析蛋白质互作情况，根据关键蛋白的作用，分析630 nm LED红光照射与THP-1来源M0巨噬细胞的关系。结果 蛋白质组学结果显示，630 nm LED红光照射THP-1来源M0巨噬细胞后，62个差异表达蛋白能够显著富集在细胞对刺激反应、线粒体膜电势负性调控和平滑肌细胞增殖的调控等相关生物学进程。在显著性排名前10的生物学进程中，细胞对刺激反应相关生物学进程占70%,主要为对糖刺激反应(4个进程)、对神经生长因子反应(2个进程)等；进一步分析细胞应对刺激相关生物学进程中涉及的8个蛋白间蛋白互作情况结果显示，转录因子叉头箱蛋白O3亚基(forkhead box O3,FOXO3)、炎症相关蛋白前列腺素内过氧化物合酶2(prostaglandin-endoperoxide synthase 2,PTGS2)或称环加氧酶2(cyclooxygenase-2,COX-2)和炎症因子白介素-18(interleukin-18,IL-18)为关键互作蛋白，并且在630 nm LED红光照射后的THP-1来源M0巨噬细胞中，FOXO3和IL-18的蛋白水平显著升高(P<0.05),PTGS2(COX-2)蛋白水平显著降低(P<0.05)。结论 630 nm LED红光照射对THP-1来源M0巨噬细胞的作用，类似于糖和神经生长因子对细胞的刺激反应；通过上调THP-1来源M0巨噬细胞中FOXO3蛋白的表达抑制M0巨噬细胞中PTGS2(COX-2)介导的炎症状态。更多还原

【Abstract】 Objective To analyze the effect and possible mechanism of 630 nm light emitting diode(LED) red light irradiation on THP-1 derived M0 macrophage. Methods The THP-1 derived M0 macrophage was induced by phorbol-12-myristate-13-acetate(PMA) treatment from THP-1 cells and irradiated with 28 J/cm~2 630 nm LED red light. The differentially expressed protein were screen out by non-standard quantitative proteomic analysis method and were annotated and enriched into gene ontology(GO) biological processes. The protein-protein interaction analysis of the enriched proteins in the GO biological processes was achieved by string online website(https://cn.string-db.org/) and Cytoscape software, relationship between 630 nm LED red light irradiation and THP-1 derived M0 macrophages was analyzed based on the role of key proteins. Results The proteomic analysis results showed that 62 differentially expressed proteins could significantly enriched into GO biological processes in the M0 macrophage irradiated with 630 nm LED red light, including cellular response to stimulus, negative regulation of mitochondrial membrane potential and regulation of smooth muscle cell proliferation, and so on. Seven biological processes(70%) of the response to stimulus were selected among the top 10 biological processes according to significance, including response to hexose(4 processes) and response to nerve growth factor(2 processes). There were 8 proteins involved in the cellular response to stimulus, and the forkhead box O3(FOXO3), prostaglandin-endoperoxide synthase 2(PTGS2) or cyclooxygenase-2(COX-2) and interleukin-18(IL-18) were identified as key interacting proteins with protein-protein interaction analysis. The expression level of the transcriptional factor FOXO3 and inflammatory factor IL-18 were elevated, and expression level of the inflammatory protein PTGS2(COX-2) was decreased in the cells after irradiation of 630 nm LED red light(P<0.05). Conclusion The irradiation of 630 nm LED red light is considered similar to the stimulation of the hexose and nerve growth factor, and presents anti-inflammation effect mediated by PTGS2(COX-2) in THP-1 derived M0 macrophage through up-regulating of FOXO3 protein.更多还原