【摘要】 目的 研究桑枝多糖对肾损伤高尿酸血症大鼠尿酸水平的影响及作用机制。方法 将40只SD大鼠随机均分为空白组、模型组、阳性（非布司他）组和桑枝多糖高、低剂量(1.2、0.6 g·kg^-1)组。从第7天起，仅空白组继续饲喂普通饲料，其余组大鼠饲喂2.5%氧嗪酸钾和0.25%腺嘌呤并给药。第35天时，给药1 h后，眼球取血，测定血清尿酸（SUA）、肌酐（SCr）及尿素氮（BUN）的含量。第42天时，于末次给药后，股动脉取血，测定血清SUA、SCr及BUN的含量；收集肾组织制成切片，HE染色观察各组大鼠的肾病理情况；采用免疫组织化学方法测定Ⅰ型胶原蛋白（ColⅠ）和三磷酸腺苷结合转运蛋白2（ABCG2）的表达水平。结果 与模型组比较，桑枝多糖高剂量能显著降低高尿酸血症大鼠血清SUA、BUN和Scr的含量，减少肾小管棕褐色尿酸盐晶体，增加ABCG2蛋白的表达，降低ColⅠ蛋白的表达。结论 桑枝多糖对高尿酸血症大鼠具有降尿酸作用，其机制与加强ABCG2蛋白的表达有关；RMP通过抑制ColⅠ蛋白的表达，减缓肾脏纤维化进展，对肾脏有一定的保护作用。更多还原

【Abstract】 OBJECTIVE To investigate the effect of Ramulus mori polysaccharides（RMP） on uric acid level and its mechanism of action in hyperuricemia rats with renal injury.METHODS SD rats（n=40） were randomly divided into the blank group, model group, positive（febuxostat） group, high dose(1.2 g·kg^-1) of RMP group and low dose(0.6 g·kg^-1) of RMP groups.From day 7,all the groups were fed a 2.5% potassium oxonate and 0.25% adenine diet, except that the blank group was fed a normal diet.The drug treatment began at the same time every day for 35 days.The blood was taken from the eye socket, and the SUA,SCr, and BUN were measured on the 35th day.Femoral arterial blood was collected on the last day.SUA,SCr, and BUN were measured.Kidney tissues were collected for histopathological analysis（HE staining）.Immunohistochemical method was used to evaluate the protein expression level of ABCG2 and ColⅠin the rat kidney.RESULTS Compared with the model group, the contents of serum SUA,BUN and Scr in hyperuricemia rats were significantly reduced by high dose RMP significantly.Pathological sections by HE staining showed remarkably decreased urate crystals in renal tubules of the low and high dose of RMP groups as compared with the model group.The level of ABCG2 was increased and the level of ColⅠwas reduced in the high dose group of RMP.CONCLUSION RMP can reduce the uric acid level in a hyperuricemia rat model, and its mechanism is related to the level of ABCG2 protein expression level.RMP can protect the kidney by inhibiting the expression of the ColⅠprotein.更多还原