【摘要】 目的 筛选具有保护人皮肤角质形成细胞（HACAT）抗过氧化氢（H₂O₂）氧化损伤的活性样品，并探索其抗氧化的机制。方法 用CCK-8法测定不同植物样品对H₂O₂损伤HACAT细胞的保护作用，获得活性样品远志寡精F。检测远志寡精F对损伤细胞的增殖活性、超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-Px）的活性、丙二醛（MDA）、活性氧（ROS）、白细胞介素（IL）-6、IL-8的含量以及细胞凋亡和坏死的影响。结果 经筛选得到的活性物质远志寡精F能提高损伤细胞的增殖活力、SOD及GSH-Px活性，减少MDA、ROS的产生和IL-6、IL-8的表达，降低细胞的凋亡率和坏死率。结论 远志寡精F可能是通过增加抗氧化活性物质的产生、减轻炎症反应、减少细胞凋亡和坏死来发挥抗氧化损伤的作用。更多还原

【Abstract】 OBJECTIVE To screen the active samples which can protect HACAT cell against H₂O₂ oxidative damage, and to explore its mechanism.METHODS The protective effects of different plant samples on H₂O₂ damaged HACAT cells were determined by CCK-8 to obtain the active samples.The effect of active samples on proliferation, activities of SOD and GSH-Px, contents of MDA,ROS,IL-6 and IL-8,apoptosis and necrosis of injured cells were detected.RESULTS Tenuifolioses F was obtained by screening.It can increase the proliferation, activities of SOD and GSH-Px, decrease the levels of MDA,ROS,IL-6 and IL-8,reduce the apoptosis and necrosis of injured HACAT cells.CONClUSION Tenuifolioses F may play an anti-oxidative injury role by increasing antioxidant active substances, reducing inflammatory response, and reducing cell apoptosis and necrosis.更多还原