【摘要】 本文利用聚多巴胺-氧化石墨烯（PDA-GO）复合材料和纳米金颗粒（AuNPs）修饰电极,构建了用于17β-雌二醇（E2）灵敏检测的电化学生物传感器。PDA-GO复合膜可显著加速电子转移,增大活性面积,表现出优异的电化学性能。该研究通过在电极上组装适配体和互补DNA,形成基于双链结构的传感界面,以杂交指示剂亚甲基蓝（MB）为信号探针,进行信号放大。目标物E2存在时,E2与适配体特异性结合导致适配体从电极表面解离,双链解开释放MB,进而通过测量MB的电信号,实现对E2的灵敏检测。构建的电化学生物传感器对E2的线性范围为5.0×10^-11～1.0×10^-7M,检出限为3.0×10^-11 M。该方法具有较高的灵敏度和选择性,可用于环境样品中雌激素的生物分析。更多还原

【Abstract】 Herein,an electrochemical aptasensor for the sensitive detection of 17-estradiol（E2） was constructed using polydopamine-graphene oxide（PDA-GO） composite and gold nanoparticle（AuNPs） modified electrode.PDA-GO composite can significantly accelerate electron transfer and increase the active area,showing excellent electrochemical performance.In this study,aptamer and complementary DNA were assembled on the electrode to form a sensing interface based on double-stranded structure,and the hybridization indicator methylene blue（MB） was used as a signal probe for signal amplification.When the target E2 was present,the specific binding of E2 to the aptamer resulted in the dissociation of the aptamer from the electrode surface and the release of MB from the double chain.Then,the sensitive detection of E2 can be realized by measuring the signal of MB.The linear range of E2 of the aptasensor was 5.0×10^-11～1.0×10^-7 M,and the detection limit was 3.0×10^-11 M.The method demonstrated high sensitivity and selectivity,and could be used for the biological analysis of estrogen in environmental samples.更多还原