【摘要】 本文介绍了一种基于无标记双重信号放大的简单、超灵敏的配体传感器，采用电化学阻抗法检测凝血酶，用氧化还原探针[Fe（CN）₆]^3-/4-分析表征电极表面电荷传递阻抗的变化(ΔR_et),凝血酶与其适配体特异性结合，形成的“三明治”夹心结构，以及双链DNA刚性结构的增敏作用，可实现双重放大信号的作用，提高目标物的测定灵敏度。结果显示，线性范围良好，为0.02～10 nmol·L^-1,检出限低，达0.22 pmol·L^-1,方法简单、灵敏、特异性高，能用于人血清样品中凝血酶的检测。更多还原

【Abstract】 In this paper, a simple and ultra-sensitive thrombin aptasensor based on unlabeled double signal amplification is introduced.Thrombin was detected by electrochemical impedance method, and the change of electron transfer resistance(ΔR_et)on the electrode surface is characterized by REDOX probe[Fe（CN）₆]^3-/4-.The sandwich structure formed by the specific binding of thrombin to its aptamer and the sensitization effect of the rigid structure of double-stranded DNA can achieve the function of double amplification signal and improve the sensitivity of the target determination.The linear range of the method was 0.02～10 nmol·L^-1,and the detection limit was 0.22 pmol·L^-1.The method was simple, sensitive and specific, and could be used for the detection of actual samples.更多还原