【摘要】 用三磷酸腺苷（Adenosine 3-triphosphate简称ATP）适配体BT5A5模板稳定的BT5A5-Ag Ncs探针检测ATP。当ATP出现时，ATP与适配体特异性结合诱导ATP适配体的构象发生改变，促使DNA两端荧光弱的Ag NCs相互靠近，导致荧光增强。测定了BT5A5-Ag NCs的TEM,BT5A5-Ag NCs的粒径均匀且在2 nm左右。在最佳条件下，ATP在0～15 mmo·L^-1性范围内的检测限为19μmol·L^-1。此方法成功地检测了胎牛血清中的ATP,回收率在98.3%～103.7%的范围。因此该方法对生物样品中ATP检测具有潜在的应用价值。更多还原

【Abstract】 ATP was detected using with the probe of BT5A5-Ag NCs stabilized by ATP aptamer BT5A5 templates.In the presence of ATP,the specific conjugation of ATP and its aptamer induces the conformational change of ATP aptamer, prompts the dark fluorescence Ag NCs located in two termini of DNA close, resulting in its fluorescence enhancing.The TEM of BT5A5-Ag NCs was measured and the particle size of BT5A5-Ag NCs is uniform and about 2 nm.Under optimum conditions, a limit of detection is 19 μmol·L^-1 in the linear range of 0～15 mmol·L^-1.ATP in fetal bovine serum was detected successfully by using this proposed method with the recoveries in the range of 98.3%～103.7%.Therefore, this method has potential application value for the detection of ATP in biological samples.更多还原