【摘要】 在聚多巴胺修饰的基底表面同时接枝端基为氨基的聚(2-甲基-2-噁唑啉)和端基为巯基的聚(4-乙烯吡啶),制备了聚(2-甲基-2-噁唑啉)/聚(4-乙烯吡啶)混合刷.通过X射线光电子能谱、可变角光谱型椭偏仪、zeta电位仪和静态水接触角对涂层进行表征.结果表明,混合刷的组成可以通过改变聚(2-甲基-2-噁唑啉)和聚(4-乙烯吡啶)的投料比控制.此外,改变环境pH值和混合刷组成比例可以调节混合刷涂层表面的zeta电位和水接触角.采用异硫氰酸荧光素标记胃蛋白酶和表面等离子体共振研究了胃蛋白酶在聚(2-甲基-2-噁唑啉)/聚(4-乙烯吡啶)混合刷表面的响应性吸附/脱附能力.结果表明,通过调整聚(2-甲基-2-噁唑啉)或聚(4-乙烯吡啶)的组分比,在pH=3时,聚(2-甲基-2-噁唑啉)/聚(4-乙烯吡啶)混合刷表面可以吸附大量的胃蛋白酶;在pH=7时,脱附效率达到92%以上.更多还原

【Abstract】 Mixed polymer brushes coating based on poly(2-methyl-2-oxazoline)/poly(4-vinyl pyridine)(PMOXA/P4VP)was prepared by simultaneously grafting amine-terminated PMOXA and thiolterminated P4VP onto poly(dopamine)(PDA)-modified substrates in this work.The coatings were characterized by X-ray photoelectron spectroscopy,ellipsometry,zeta potential measurements,and the static water contact angle tests.The results indicated that it is feasible to control the components of the coating by adjusting the feed ratio of PMOXA to P4VP.Moreover,the zeta potential and the water contact angle of mixed brushes modified surfaces could be tuned by changing the environmental pH value and surface compositions.Finally,fluorescein isothiocyanate-labelled pepsin assay and surface plasmon resonance were performed to investigate the responsive adsorption/desorption of pepsin by PMOXA/P4VP mixed brushes.The results showed that by adjusting the fraction of PMOXA or P4VP,the PMOXA/P4VP mixed brushes coated surfaces could adsorb a high amount of pepsin at pH=3,and achieve a desorption efficiency of over 92% at pH=7.更多还原