【摘要】 利用兰州重离子研究装置(HIRFL)的碳离子束辐照油葵干种子,统计出苗率、存活率、花粉活力、柱头可授性和不同授粉条件下的结实率,研究碳离子束辐照对油葵当代结实特性的影响。结果表明,出苗率、存活率、花粉活力、结实率随着辐照剂量增加而降低;与对照相比,80 Gy辐照后出苗率下降55.68%、存活率下降64.66%;20～160 Gy辐照显著降低了自交结实率,40～160 Gy辐照显著降低了混交结实率;花粉活力由对照组的92.01%降低为160 Gy的47.59%;随着辐照剂量的增加,具有较强可授性柱头的百分比逐渐降低,具有较弱可授性柱头和不具可授性柱头的百分比逐渐增加。研究表明,碳离子束辐照对油葵当代的损伤作用,影响了花器官的育性,降低了结实率;根据存活率的半致死剂量,结合混交结实率的半不育剂量,确定油葵适宜辐照剂量范围55～153 Gy,为碳离子束辐照油葵育种选择适宜辐照剂量提供了参考。更多还原

【Abstract】 The dry seeds of oil sunflower were irradiated with the carbon ion beam provided by the Heavy Ion Research Facility(HIRFL) in Lanzhou.To research the effects of carbon ion beam irradiation on the fruiting characters of M1 oil-sunflower,the seedling emergence rate,survival rate,pollen viability,stigma receptivity and seed setting rate under different pollination conditions were measured.The results showed that the seedling emergence rate,survival rate,pollen viability and seed setting rate reduced with the irradiation dose increasing.Compared with control,the emergence rate and survival rate of oil-sunflower decreased by 55.68% and 64.66% after seeds irradiated with a carbon ion dose of 80 Gy.This study also revealed that seeds were irradiated with 20～160 Gy showed very low self-cross seed setting rate,and the mixed-cross seed setting rate were significantly reduced at irradiation of 40～160 Gy.Pollen viability decreased from 92.01% of the CK to 47.59% of the 160 Gy irradiation.With the irradiation dose increasing,the percentage of stigmas with higher receptivity reduced,and the percentage of stigmas with lower receptivity and no receptivity increased.All results indicated that the damage of carbon ion beam irradiation inhibited the fertility of flower organs and reduced the seeding rate.According to the semi-lethal dose of survival rate and the semi-sterile dose of mixed-cross seed setting rate,the recommended irradiation dose range of oil sunflower is 55 to 153 Gy.Our findings may lead to more reference for the selection of suitable irradiation dose for oil sunflower breeding by carbon ion beam irradiation.更多还原