【摘要】 为建立坛紫菜丝状体阶段良种选育的筛选指标，以坛紫菜选育品系（GS-1）、闽丰2号（MF-2）和福建野生种（FJ-W18）的丝状体为材料，检测其在20、25、30、32℃下的生长、光合作用和光合色素等相关参数，并重点关注自由丝状体的钻壳效率和贝壳丝状体壳孢子放散量等生产性状。结果表明：GS-1、MF-2和FJ-W18丝状体在不同温度下相对生长率大小依次为20℃>25℃>30℃>32℃，在20℃时生长最佳；25℃时GS-1相对生长率为1.5%，极显著高于MF-2(0.45%)和FJ-W18(0.79%)(P<0.01)；超过30℃时各品系生长明显受抑制。随温度升高、时间延长，各品系的丝状体最大光化学量子产量（Fv/Fm）、叶绿素a和藻胆蛋白含量都呈下降趋势；且GS-1的叶绿素a含量随温度、时间变化均高于MF-2和FJ-W18。以初始藻落密度来评价自由丝状体的钻壳效率，GS-1的初始藻落密度是MF-2的1.38倍，是FJ-W18的1.71倍；贝壳丝状体在不同温度扩增培养后，相同条件诱导壳孢子囊枝并放散壳孢子，GS-1壳孢子放散量均高于MF-2和FJ-W18。综上可知，GS-1丝状体具有较快的生长速率，较好的高温抗性以及优良的钻壳效率和壳孢子放散量，具有高抗性、高繁殖力良种开发的潜力。本研究为坛紫菜新品系的优选和人工培育提供了数据和理论参考。更多还原

【Abstract】 In order to establish screening indexes for genetic breeding of Neoporphyra haitanensis at the conchocelis stage, the growth rate, photosynthetic parameters, and photosynthetic pigments were investigated on the conchocelis of N. haitanensis cultivars like GS-1(breeding cultivar), MF-2(cultivar Minfeng-2), and FJ-W18(wild type from Fujian coast) at 20, 25, 30 and 32 ℃. The shell drilling efficiency of free-living conchocelis and the amount of conchospores released of shell-borne conchocelis were emphasized. The results showed that: The relative growth rates of GS-1, MF-2, and FJ-W18 conchocelis at different temperatures were: 20 ℃>25 ℃>30 ℃>32 ℃, with 20 ℃ showing the best growth. The relative growth rate of GS-1 was 1. 5% at 25 ℃, which was extremely significantly higher than that of MF-2(0. 45%) and FJ-W18(0. 79%)(P<0. 01). Growth was obviously impeded above 30 ℃ for all cultivars. The maximum photochemical quantum yield(Fv/Fm), contents of chlorophyll a and phycobiliproteins of each cultivar decreased as temperature and time increased. However, the increased chlorophyll a content of GS-1 with higher temperature and longer time were more than those of MF-2 and FJ-W18. The shell drilling effectiveness of free-living conchocelis was assessed using the initial algal colony density. GS-1 had the highest initial colony density, which were 1. 38times that of MF-2 and 1. 71 times that of FJ-W18. The shell-borne conchocelis was amplified under different temperatures, after that, the conchosporangium were introduced and the conchospores were released at the same conditions. The results showed that GS-1 had a larger discharge of conchospores than MF-2 and FJ-W18.Based on the above indicators, GS-1 conchocelis has fast growth rate, good high temperature resistance, excellent shell drilling efficiency and conchospore dispersion, which has the potential to develop improved cultivar with high reproduction capacity and high resistance. The results provided data and theoretical basis for new cultivar selection and artificial cultivation of N. haitanensis.更多还原